Department of Cell & Systems Biology, University of Toronto, 25 Willcocks Street, Toronto, ON M5S 3B2, Canada.
Institute of Molecular Systems Biology, ETH, Zurich, Switzerland.
Mol Cell. 2024 Oct 17;84(20):4031-4047.e11. doi: 10.1016/j.molcel.2024.09.004. Epub 2024 Oct 1.
Inter-kingdom communication through small molecules is essential to the coexistence of organisms in an ecosystem. In soil communities, the plant root is a nexus of interactions for a remarkable number of fungi and is a source of small-molecule plant hormones that shape fungal compositions. Although hormone signaling pathways are established in plants, how fungi perceive and respond to molecules is unclear because many plant-associated fungi are recalcitrant to experimentation. Here, we develop an approach using the model fungus, Saccharomyces cerevisiae, to elucidate mechanisms of fungal response to plant hormones. Two plant hormones, strigolactone and methyl jasmonate, produce unique transcript profiles in yeast, affecting phosphate and sugar metabolism, respectively. Genetic analysis in combination with structural studies suggests that SLs require the high-affinity transporter Pho84 to modulate phosphate homeostasis. The ability to study small-molecule plant hormones in a tractable genetic system should have utility in understanding fungal-plant interactions.
种间通过小分子通讯对于生态系统中生物的共存至关重要。在土壤群落中，植物根系是大量真菌相互作用的枢纽，也是小分子植物激素的来源，这些激素影响真菌的组成。尽管植物中已经建立了激素信号通路，但真菌如何感知和响应这些分子尚不清楚，因为许多与植物相关的真菌难以进行实验研究。在这里，我们使用模式真菌酿酒酵母开发了一种方法，以阐明真菌对植物激素响应的机制。两种植物激素，独脚金内酯和茉莉酸甲酯，在酵母中产生独特的转录谱，分别影响磷酸盐和糖代谢。遗传分析结合结构研究表明，SLs 需要高亲和力转运蛋白 Pho84 来调节磷酸盐稳态。在可遗传的遗传系统中研究小分子植物激素的能力应该有助于理解真菌-植物相互作用。
