Wang Yan, Zhou Changlong, Guo Qianqian, Chen Bin, Luo Jia, Lv Yimin
Oral and Maxillofacial Plastic Surgery Center, Ningbo Medical Center Lihuili Hospital (The Affiliated Lihuili Hospital of Ningbo University).
Dent Mater J. 2024 Dec 10;43(6):780-788. doi: 10.4012/dmj.2024-066. Epub 2024 Oct 2.
The study is to evaluate the effects of collagen/hyaluronic acid coating with or without puerarin and exosomes (Exos) derived from adipose stem cells (ADSCs-Exos) on pre-osteoblast proliferation and differentiation on the surface of titanium materials. Titanium materials with different coatings were prepared by layer-by-layer technique, evaluating the surface characterization. Cell functions were assessed by cell biology experiments. Related genes and proteins were assessed by RT-qPCR and Western blot. Puerarin or ADSCs-Exos coating had better effects on promoting the adhesion, proliferation and differentiation of pre-osteoblasts, and the strongest effect was found after their co-coatings, manifesting as the up-regulations of alkaline phosphatase (ALP) activity, collagen type I alpha 1 (Col1a1), runt-related transcription factor 2 (Runx2), osterix and activating transcription factor-2 (ATF-2). Levels of phosphorylated-P38 (p-P38) and p-ATF-2 were up-regulated in pre-osteoblasts grown on puerarin and ADSCs-Exos-loaded titanium surfaces. Titanium surfaces loaded with puerarin and ADSCs-Exos promotes the proliferation and differentiation of pre-osteoblasts.
本研究旨在评估含或不含葛根素及脂肪干细胞来源的外泌体（ADSCs-Exos）的胶原蛋白/透明质酸涂层对钛材料表面前成骨细胞增殖和分化的影响。采用层层技术制备了具有不同涂层的钛材料，并对其表面特性进行了评估。通过细胞生物学实验评估细胞功能。通过RT-qPCR和蛋白质印迹法评估相关基因和蛋白质。葛根素或ADSCs-Exos涂层对促进前成骨细胞的黏附、增殖和分化具有更好的效果，两者共涂层后效果最强，表现为碱性磷酸酶（ALP）活性、I型胶原α1（Col1a1）、 runt相关转录因子2（Runx2）、osterix和激活转录因子-2（ATF-2）的上调。在负载葛根素和ADSCs-Exos的钛表面生长的前成骨细胞中，磷酸化-P38（p-P38）和p-ATF-2的水平上调。负载葛根素和ADSCs-Exos的钛表面促进前成骨细胞的增殖和分化。
