Matsumoto Riko, Obata Shumpei, Kakinoki Masashi, Sawada Osamu, Kawamoto Ikuo, Murase Mitsuru, Ohji Masahito
Department of Ophthalmology, Shiga University of Medical Science, Otsu, Japan.
Research Center for Animal Life Science, Shiga University of Medical Science, Otsu, Japan.
J Ocul Pharmacol Ther. 2025 Jan-Feb;41(1):33-38. doi: 10.1089/jop.2024.0138. Epub 2024 Oct 2.
To evaluate the duration of vascular endothelial growth factor (VEGF) suppression in the aqueous humor of macaque eyes after intravitreal faricimab (IVF) injection. Faricimab (6 mg/50 µL) was injected into the vitreous cavity of the right eye of 6 macaques. Aqueous humor samples (150 μL) were collected from both eyes immediately before injection and on days 1, 3, 7, 14, 21, 28, 42, 56, 84, and 112 after injection. The VEGF concentrations in the aqueous humor were measured using an enzyme-linked immunosorbent assay. The VEGF was undetectable until 4 weeks after IVF injection in 4 eyes and until 6 weeks in the remaining 2 eyes. The mean duration of complete VEGF suppression was 4.7 weeks (range, 4-6 weeks). The VEGF concentration did not decrease in the aqueous humor of the non-injected fellow eyes. Faricimab effectively suppressed the VEGF concentrations in the aqueous humor of macaques for an average of 4.7 weeks after a single intravitreal injection. It did not reduce the VEGF concentrations in the aqueous humor of the fellow eyes.
评估玻璃体内注射法瑞西单抗（IVF）后猕猴眼房水中血管内皮生长因子（VEGF）抑制的持续时间。将法瑞西单抗（6 mg/50 µL）注射到6只猕猴右眼的玻璃体腔中。在注射前以及注射后第1、3、7、14、21、28、42、56、84和112天，从双眼采集房水样本（150 μL）。使用酶联免疫吸附测定法测量房水中的VEGF浓度。4只眼在IVF注射后4周内VEGF检测不到，其余2只眼在6周内检测不到。VEGF完全抑制的平均持续时间为4.7周（范围为4 - 6周）。未注射的对侧眼房水中的VEGF浓度没有降低。单次玻璃体内注射后，法瑞西单抗可有效抑制猕猴房水中的VEGF浓度，平均持续4.7周。它不会降低对侧眼房水中的VEGF浓度。
