Department of Procurement Management, Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing, China.
Key Laboratory for Tibet Plateau Phytochemistry of Qinghai Province, Qinghai Minzu University-Zangnuo Joint-Lab, College of Pharmacy, Qinghai Nationalities University, Xining, China.
Luminescence. 2024 Oct;39(10):e4910. doi: 10.1002/bio.4910.
A novel peptide-based chemical fluorescence sensor L (Dansyl-His-Pro-Thr-Cys-NH) was designed and synthesized. This sensor exhibits an "On-Off-On" detection cycle to detect Cu, Zn, and S in solution. According to the chelation-enhanced fluorescence (CHEF) mechanism, when Zn is present, the fluorescence is significantly enhanced and a blue shift occurs, representing a "Turn-On" phase of the fluorescence detection mode. Because copper ions (Cu) have a paramagnetic quenching sensing mechanism, the fluorescence of L quenches rapidly with the formation of the L-Cu system, representing the "Turn-Off" phase. The subsequent introduction of S to the L-Cu system results in the recovery of the L-fluorescence, thereby representing the second "Turn-On" phase. As a peptide molecule, the sensor L has several advantages over other types of sensors, including water solubility, high sensitivity, and good biocompatibility, with a very low detection limit. The detection lines of Zn and Cu are 97 nM (R = 0.993) and 75 nM (R = 0.995), respectively. Additionally, the sensor does not exhibit any obvious cell toxicity. These results indicate that this peptide chemiluminescent sensor has the potential to be applied in in vivo detection.
一种新型的基于肽的化学荧光传感器 L（Dansyl-His-Pro-Thr-Cys-NH）被设计和合成。该传感器对溶液中的 Cu、Zn 和 S 具有“开-关-开”的检测循环。根据螯合增强荧光（CHEF）机制，当存在 Zn 时，荧光显著增强且发生蓝移，代表荧光检测模式的“开启”阶段。由于铜离子（Cu）具有顺磁猝灭传感机制，L 与 Cu 形成 L-Cu 体系时，荧光迅速猝灭，代表“关闭”阶段。随后将 S 引入 L-Cu 体系，导致 L-荧光恢复，从而代表第二个“开启”阶段。作为一种肽分子，传感器 L 具有其他类型传感器所不具备的几个优点，包括水溶性、高灵敏度和良好的生物相容性，且检测限非常低。Zn 和 Cu 的检测线分别为 97 nM（R=0.993）和 75 nM（R=0.995）。此外，该传感器没有表现出明显的细胞毒性。这些结果表明，这种肽化学发光传感器具有在体内检测中的应用潜力。
