Xue Yamei, Zheng Haixia, Xiong Yuping, Li Kun
Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Institute for Reproductive Health, School of Pharmacy, Hangzhou Medical College, Hangzhou, China.
Front Pharmacol. 2024 Sep 18;15:1366992. doi: 10.3389/fphar.2024.1366992. eCollection 2024.
Extracellular vesicles (EVs) are nanometer-sized lipid bilayer vesicles released by cells, playing a crucial role in mediating cellular communication. This review evaluates the effect of EVs on early embryonic development by systematically searching the literature across three databases, Embase, PubMed, and Scopus, from inception (Embase, 1947; PubMed, 1996; and Scopus, 2004) to 30 June 2024. A total of 28 studies were considered relevant and included in this review. The EVs included in these investigations have been recovered from a range of sources, including oviduct fluid, follicular fluid, uterine fluid, seminal plasma, embryos, oviduct epithelial cells, endometrial epithelial cells, amniotic cells, and endometrial-derived mesenchymal stem cells collected primarily from mice, rabbits, cattle and pigs. This diversity in EV sources highlights the broad interest and potential applications of EVs in embryo culture systems. These studies have demonstrated that supplementation with EVs derived from physiologically normal biofluids and cells to the embryo culture medium system has positive effects on embryonic development. Conversely, EVs derived from cells under pathological conditions have shown a negative impact. This finding underscores the importance of the source and condition of EVs used in culture media. Further, the addition of EVs as a culture medium supplement holds significant therapeutic potential for optimizing embryo culture systems. In conclusion, this evaluation offers a thorough assessment of the available data on the role of EVs in embryo culture media and highlights the potential and challenges of using EVs in embryo production.
细胞外囊泡（EVs）是细胞释放的纳米级脂质双层囊泡，在介导细胞通讯中发挥着关键作用。本综述通过系统检索Embase、PubMed和Scopus这三个数据库，从建库起始（Embase为1947年；PubMed为1996年；Scopus为2004年）到2024年6月30日的文献，评估了细胞外囊泡对早期胚胎发育的影响。共有28项研究被认为相关并纳入本综述。这些研究中涉及的细胞外囊泡来源广泛，包括输卵管液、卵泡液、子宫液、精浆、胚胎、输卵管上皮细胞、子宫内膜上皮细胞、羊膜细胞以及主要从小鼠、兔子、牛和猪收集的子宫内膜来源的间充质干细胞。细胞外囊泡来源的这种多样性凸显了其在胚胎培养系统中的广泛关注和潜在应用。这些研究表明，向胚胎培养基系统中添加源自生理正常生物流体和细胞的细胞外囊泡对胚胎发育具有积极影响。相反，源自病理条件下细胞的细胞外囊泡则显示出负面影响。这一发现强调了用于培养基的细胞外囊泡来源和条件的重要性。此外，添加细胞外囊泡作为培养基补充剂在优化胚胎培养系统方面具有巨大的治疗潜力。总之，本评估全面评估了关于细胞外囊泡在胚胎培养基中作用的现有数据，并突出了在胚胎生产中使用细胞外囊泡的潜力和挑战。
