A synonymous mutation of rs1137070 cause the mice gene transcription and translation to decrease.

The Monoamine Oxidase-A () EcoRV polymorphism (rs1137070) is a unique synonymous mutation (c.1409 T > C) within the gene, which plays a crucial role in gene expression and function. This study aimed to explore the relationship between the mouse rs1137070 genotype and differences in gene expression. Mice carrying the CC genotype of rs1137070 exhibited a significantly lower expression level, with an odds ratio of 2.44 compared to the T carriers. Moreover, the wild-type TT genotype of MAOA demonstrated elevated mRNA expression and a longer half-life. We also delved into the significant expression and structural disparities among genotypes. Furthermore, it was evident that different aspartic acid synonymous codons within influenced both expression and enzyme activity, highlighting the association between rs1137070 and MAOA. To substantiate these findings, a dual-luciferase reporter assay confirmed that GAC was more efficient than GAT binding. Conversely, the synonymous mutation altered gene expression in individual mice. An RNA pull-down assay suggested that this alteration could impact the interaction with RNA-binding proteins. In summary, our results illustrate that synonymous mutations can indeed regulate the downregulation of gene expression, leading to changes in MAOA function and their potential association with neurological-related diseases.