鉴定蛋白质表达途径及用于治疗人乳头瘤病毒诱导的宫颈癌的有效微小RNA：计算机模拟分析-实验研究
Identification of proteins' expression pathway and the effective miRNAs for the treatment of human papillomavirus-induced cervical cancer: in-silico analyses-experimental research.
作者信息
Monemi Marzieh, Garrosi Lida, Mirzaei Samira, Farhadi Bahar, Ataee Disfani Reza, Zabihi Mohammad Reza, Akhoondian Mohammad, Ghorbani Vajargah Pooyan, Khorshid Alireza, Karkhah Samad
机构信息
Department of Basic Science, Faculty of Pharmacy and Pharmaceutical Science, Tehran Medical Science, Islamic Azad University, Tehran.
Department of Obstetrics and Gynecology, Zanjan University of Medical Sciences, Zanjan.
出版信息
Ann Med Surg (Lond). 2024 Aug 30;86(10):5784-5792. doi: 10.1097/MS9.0000000000002513. eCollection 2024 Oct.
INTRODUCTION
Cervical cancer is the fourth most common cancer in women. The risk factors for cervical cancer include human papillomavirus (HPV) infection, age, smoking, number of pregnancies, use of oral contraceptives, and diet. However, long-term HPV infection appears to be the main risk factor for developing cervical cancer. This in-silico analysis aims to identify the expression network of proteins and the miRNAs that play a role in the development of HPV-induced cervical cancer.
METHODS
The critical proteins and miRNAs were extracted using the DisGeNET and miRBase databases. String and Gephi were applied to the network analysis. The GTEx web tool was utilized to Identify tissue expression levels. The Enrichr website was used to explore the molecular function and pathways of found genes.
RESULTS
Ten proteins, TP53, MYC, AKT1, TNF, IL6, EGFR, STAT3, CTNNB1, ESR1, and JUN, were identified as the most critical shared gene network among cervical cancer and HPV. Seven miRNAs were found, including hsa-mir-146a, hsa-mir-27, hsa-mir-203, hsa-mir-126, hsa-mir-145, hsa-mir-944, and hsa-mir-93, which have a common expression in cervical cancer and HPV.
CONCLUSION
Overall, the gene network, including TP53, MYC, AKT1, TNF, IL6, EGFR, STAT3, CTNNB1, ESR1, and JUN, and Also, hsa-mir-145, hsa-mir-93, hsa-mir-203, and hsa-mir-126 can be regarded as a gene expression pathway in HPV-induced cervical cancer.
引言
宫颈癌是女性中第四大常见癌症。宫颈癌的风险因素包括人乳头瘤病毒（HPV）感染、年龄、吸烟、怀孕次数、口服避孕药的使用以及饮食。然而，长期HPV感染似乎是患宫颈癌的主要风险因素。这项计算机模拟分析旨在识别在HPV诱导的宫颈癌发展过程中起作用的蛋白质和微小RNA（miRNA）的表达网络。
方法
使用DisGeNET和miRBase数据库提取关键蛋白质和miRNA。将String和Gephi应用于网络分析。利用GTEx网络工具确定组织表达水平。使用Enrichr网站探索所发现基因的分子功能和途径。
结果
十种蛋白质，即TP53、MYC、AKT1、TNF、IL6、EGFR、STAT3、CTNNB1、ESR1和JUN，被确定为宫颈癌和HPV之间最关键的共享基因网络。发现了七种miRNA，包括hsa - mir - 146a、hsa - mir - 27、hsa - mir - 203、hsa - mir - 126、hsa - mir - 145、hsa - mir - 944和hsa - mir - 93，它们在宫颈癌和HPV中具有共同表达。
结论
总体而言，包括TP53、MYC、AKT1、TNF、IL6、EGFR、STAT3、CTNNB1、ESR1和JUN的基因网络，以及hsa - mir - 145、hsa - mir - 93、hsa - mir - 203和hsa - mir - 126可被视为HPV诱导的宫颈癌中的基因表达途径。
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