Merchenthaler I, Arimura A
Peptides. 1985 Sep-Oct;6(5):865-7. doi: 10.1016/0196-9781(85)90316-x.
The effect of removal of CH by long-term hypophysectomy (HPX) on the GHRF-immunoreactivity in the rat hypothalamus was studied using immunocytochemical methods. HPX resulted in a dramatic reduction in immunostaining in the median eminence (ME), indicating a significant reduction in storage of GHRF. Other regions of the hypothalamus, including the dorsomedial nucleus, the medial preoptic and suprachiasmatic regions, and the lateral hypothalamus showed the distribution and intensity of GHRF-immunoreactive structures as seen in intact animals; i.e., scattered fibers only. A few weakly stained cell bodies were seen in experimental and control animals. This could be due to a lack of storage, and/or this peptide may possibly not be present in its matured and fully immunoreactive form in the cell bodies.
采用免疫细胞化学方法研究了长期垂体切除（HPX）去除CH对大鼠下丘脑生长激素释放因子（GHRF）免疫反应性的影响。垂体切除导致正中隆起（ME）免疫染色显著减少，表明GHRF的储存量显著降低。下丘脑的其他区域，包括背内侧核、内侧视前区和视交叉上区以及下丘脑外侧区，显示出与完整动物中所见的GHRF免疫反应性结构的分布和强度相同；即只有散在的纤维。在实验动物和对照动物中均可见少数弱染色的细胞体。这可能是由于缺乏储存，和/或该肽可能在细胞体中不存在成熟且完全免疫反应性的形式。
