从公牛精浆中分离碱性顶体蛋白酶抑制剂（BUSI II）。

Isolation of basic acrosin inhibitor from bull seminal plasma (BUSI II).

作者信息

Cechová D, Jonáková V, Sedláková E, Mach O

出版信息

Hoppe Seylers Z Physiol Chem. 1979 Dec;360(12):1753-8. doi: 10.1515/bchm2.1979.360.2.1753.

DOI:10.1515/bchm2.1979.360.2.1753

Abstract

An acrosin inhibitor was isolated from bull seminal plasma by gel filtration on Sephadex G-50 fine and ion-exchange chromatography on CM-Sephadex. The inhibitor is a basic polypeptide (pl greater than or equal to 10.5) of molecular weight 6 200 (calculated from amino acid composition). Its N-terminal amino group is blocked. The inhibitor is not strictly specific in its effect since it also inhibits trypsin and to a lesser degree chymotrypsin, in addition to bull and boar acrosin.

摘要

通过在Sephadex G - 50细柱上的凝胶过滤和在CM - Sephadex上的离子交换色谱法，从公牛精浆中分离出一种顶体蛋白酶抑制剂。该抑制剂是一种碱性多肽（等电点大于或等于10.5），分子量为6200（由氨基酸组成计算得出）。其N端氨基被封闭。该抑制剂的作用并非严格特异，因为除了公牛和公猪的顶体蛋白酶外，它还能抑制胰蛋白酶，对胰凝乳蛋白酶的抑制作用较弱。