Fernandes E Oliveira Hiskell Francine, Addison Owen, Yogui Fernanda C, Cruz Marcos Antonio Eufrásio, Nogueira Lucas Fabricio Bahia, Okamoto Roberta, Souza Ana Carla Gonçales, Ramos Ana Paula, Verri Fellippo Ramos
Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (Unesp), Araçatuba, Brazil.
Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
J Biomed Mater Res A. 2025 Jan;113(1):e37782. doi: 10.1002/jbm.a.37782. Epub 2024 Oct 3.
Surface treatments play an important role in enhancing the osseointegration of Titanium (Ti) and its alloys. This study introduces a method employing biomimetic hydroxyapatite (Hap) deposition guided by molecularly organized phospholipids, affixed to the metal implant surface. Using the Langmuir-Blodgett technique, phospholipids were deposited onto Ti-screws by using CaCl or CaCl/SrCl aqueous solution in the subphase of a Langmuir trough in the target proportion (i.e. 10 and 90 mol% of Sr in relation of Ca) followed by immersion in phosphate buffer and in supersaturated simulated body fluid. Coating composition and morphology were evaluated using infrared spectroscopy and scanning electron microscopy, respectively, while contact angle measurements assessed coating wettability and surface energy. Randomized screws were then implanted into the tibias of healthy and osteoporotic female rats (G1: Control-Machined, G2: Hap, G3: HapSr10, G4: HapSr90). Osseointegration, assessed 60 days post-implantation, included reverse torque, fluorochrome area, bone tissue-screw contact area, and linear extent of bone-screw contact. Results, grouped by surface treatment (Machined, Hap, HapSr10, HapSr90), revealed that the deposition of Hap, HapSr10, and HapSr90 resulted in thin and rough coatings composed of hydroxyapatite (Hap) on the screw surface with nanoscale pores. The coatings resulted in increased wettability and surface energy of Ti surfaces. The minerals are chemically similar to natural bone apatite as revealed by FTIR analysis. In vivo analyses indicated higher torque values for strontium-containing surfaces in the osteoporotic group (p = 0.02) and, in the control group superior torque for screw removal on the Hap surface (p = 0.023). Hydroxyapatite-treated surfaces enhance morphology, composition, and reactivity, promoting screw osseointegration in healthy and osteoporotic female rats. The incorporation of strontium into the mineral phase has been proposed to not only stimulate osteoblast activity but also reduce osteoclastic resorption, which may explain the improved outcomes observed here in experimental osteoporotic conditions.
表面处理在增强钛（Ti）及其合金的骨整合方面发挥着重要作用。本研究介绍了一种利用分子有序磷脂引导仿生羟基磷灰石（Hap）沉积的方法，该磷脂固定在金属植入物表面。使用朗缪尔-布洛杰特技术，通过在朗缪尔槽的亚相中以目标比例（即相对于钙，锶的摩尔百分比为10%和90%）使用氯化钙或氯化钙/氯化锶水溶液，将磷脂沉积到钛螺钉上，随后将其浸入磷酸盐缓冲液和过饱和模拟体液中。分别使用红外光谱和扫描电子显微镜评估涂层的组成和形态，同时通过接触角测量评估涂层的润湿性和表面能。然后将随机分组的螺钉植入健康和骨质疏松雌性大鼠的胫骨中（G1：对照-机械加工，G2：Hap，G3：HapSr10，G4：HapSr90）。在植入后60天评估骨整合情况，包括反向扭矩、荧光染料面积、骨组织-螺钉接触面积以及骨-螺钉接触的线性范围。按表面处理（机械加工、Hap、HapSr10、HapSr90）分组的结果显示，Hap、HapSr10和HapSr90的沉积在螺钉表面形成了由具有纳米级孔隙的羟基磷灰石（Hap）组成的薄而粗糙的涂层。这些涂层导致钛表面的润湿性和表面能增加。傅里叶变换红外光谱分析表明，这些矿物质在化学上与天然骨磷灰石相似。体内分析表明，骨质疏松组中含锶表面的扭矩值更高（p = 0.02），而在对照组中，Hap表面的螺钉移除扭矩更高（p = 0.023）。羟基磷灰石处理过的表面改善了形态、组成和反应性，促进了健康和骨质疏松雌性大鼠中螺钉的骨整合。有人提出将锶掺入矿相不仅可以刺激成骨细胞活性，还可以减少破骨细胞的吸收，这可能解释了在实验性骨质疏松条件下观察到的改善结果。
