Farazandehnia Nafiseh, Sotoudegan Farzaneh, Sepahy Abbas Akhavan, Fazeli Mohamad Reza
Department of Microbiology, Faculty of Biological Science, North of Tehran Branch, Islamic Azad University, Tehran, Iran.
Quality Control of Medicines and Supplements Group, Pharmaceutical Quality Assurance Research Center, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran.
AMB Express. 2024 Oct 3;14(1):111. doi: 10.1186/s13568-024-01759-4.
The research aimed to evaluate the antioxidative and antibacterial characteristics of aqueous sumac extract on methicillin-resistant Staphylococcus aureus through in-vitro and in-vivo study. Sumac extract has been obtained through the soaking method, and its antioxidant properties were gauged using the DPPH free radical scavenging method. The minimum inhibitory concentration (MIC) of sumac extract was determined on S. aureus obtained from hospitalized patients, as well as an assessment of biofilm-formation and the release of bacterial intracellular compounds. in vivo experimentation involved injecting bacteria (10 cfu/ml) into mice, which subsequently manifested indicators of symptoms of infection, and the number of bacteria within their bloodstream was quantified. The Sumac extract demonstrated strong antioxidant properties at concentrations of 1000 mg/ml. Furthermore, the agar tests for the gram staining, mannitol, coagulase, and DNase revealed that 190 cultured bacteria samples were identified as Staphylococcus aureus. These bacteria were resistant to clindamycin, ciprofloxacin, and methicillin antibiotics, but sensitive to erythromycin and penicillin antibiotics. Additionally, the bacteria displayed significant methicillin resistance and formed a strong biofilm (65.78%). The sumac extract showed a MIC range of 125-1000 µg/ml against Staphylococcus aureus. Treatment with concentrations above the MIC was found to prevent the formation of biofilm and increase the release of bacterial intracellular compounds. Sumac extract led to a decrease in bacterial count in the blood of mice and reduced signs of infection. Sumac extract demonstrated powerful antioxidant and antibacterial effects against resistant microorganisms, suggesting its potential as a promising compound for the treatment of resistant infections in future research.
该研究旨在通过体外和体内研究评估漆树水提取物对耐甲氧西林金黄色葡萄球菌的抗氧化和抗菌特性。漆树提取物通过浸泡法获得，其抗氧化性能采用DPPH自由基清除法进行测定。测定了漆树提取物对从住院患者分离出的金黄色葡萄球菌的最低抑菌浓度（MIC），并评估了生物膜形成和细菌细胞内化合物的释放情况。体内实验包括将细菌（10 cfu/ml）注射到小鼠体内，随后小鼠出现感染症状指标，并对其血液中的细菌数量进行定量。漆树提取物在浓度为1000 mg/ml时表现出较强的抗氧化性能。此外，革兰氏染色、甘露醇、凝固酶和DNase的琼脂试验显示，190个培养的细菌样本被鉴定为金黄色葡萄球菌。这些细菌对克林霉素、环丙沙星和甲氧西林抗生素耐药，但对红霉素和青霉素类抗生素敏感。此外，这些细菌表现出显著的耐甲氧西林特性，并形成了较强的生物膜（65.78%）。漆树提取物对金黄色葡萄球菌的MIC范围为125 - 1000 µg/ml。发现用高于MIC的浓度处理可防止生物膜形成并增加细菌细胞内化合物的释放。漆树提取物使小鼠血液中的细菌数量减少，并减轻了感染迹象。漆树提取物对耐药微生物表现出强大的抗氧化和抗菌作用，表明其在未来研究中作为治疗耐药感染的有前景化合物的潜力。
