College of Biological Science and Technology, Yili Normal University, Yining, Xin Jiang, China.
Xinjiang Key Laboratory of Lavender Conservation and Utilization at Yili Normal University, Yining, Xin Jiang, China.
PLoS One. 2024 Oct 3;19(10):e0310929. doi: 10.1371/journal.pone.0310929. eCollection 2024.
Continuous cropping is a common cultivation practice in lavender cultivation, and the structure of the soil microbial community is one of the main reasons affecting the continuous cropping disorder in lavender; however, the relationship between the number of years of cultivation and inter-root microbial composition has not yet been investigated; using Illumina high-throughput sequencing we detected fungal community structure of rhizosphere soil under 1 (L1), 3 (L3), 5 (L5) and 0 (L0) years' of lavender cultivation in Yili, Xinjiang China. The results showed that with the extension of planting years, the physical-chemical characteristics of the soil shifted, and the diversity of the fungal communities shrank, the abundance and richness of species decreased and then increased, and the phylogenetic diversity increased, The structure of the soil fungal communities varied greatly. At phylum level, dominant fungal phyla were Ascomycetes, Basidiomycetes, etc. At genus level, dominant genera were Gibberella, Mortierella, etc, whose absolute abundance all increased with increasing planting years (P < 0.05); redundancy analysis showed that thesoil physicochemical characteristics significantly correlated with dominant bacterial genera. The FUN Guild prediction showed that six groups of plant pathogens and plant saprotrophs changed significantly (P < 0.05), the amount of harmful bacteria in the soil increased while the amount of arbuscular mycorrhizal fungui (AMF) decreased, leading to a continuous cropping obstacle of lavender. The findings of this study provida theoretical foundation for the management of continuous cropping and the prevention fungus-related diseases in lavender.
连作是薰衣草栽培中的一种常见栽培方式，土壤微生物群落结构是影响薰衣草连作障碍的主要原因之一；然而，栽培年限与根际微生物组成之间的关系尚未得到研究；本研究采用 Illumina 高通量测序技术，检测了中国新疆伊犁薰衣草种植 1（L1）、3（L3）、5（L5）和 0（L0）年的根际土壤真菌群落结构。结果表明，随着种植年限的延长，土壤理化性质发生了变化，真菌群落的多样性缩小，物种的丰富度和丰度先减少后增加，系统发育多样性增加，土壤真菌群落结构发生了很大变化。在门水平上，优势真菌门为子囊菌门、担子菌门等。在属水平上，优势属为镰刀菌属、隔孢伏革菌属等，其绝对丰度均随种植年限的增加而增加（P<0.05）；冗余分析表明，土壤理化性质与优势细菌属显著相关。FUN Guild 预测表明，有六组植物病原菌和植物腐生菌发生了显著变化（P<0.05），土壤中有害细菌的数量增加，而丛枝菌根真菌（AMF）的数量减少，导致薰衣草连作障碍。本研究结果为薰衣草连作管理和防治真菌相关病害提供了理论基础。
