Guizhou Botanical Garden, Guizhou Academy of Sciences, Guiyang, Guizhou, China.
Institute of Mountain Resources of Guizhou Province, Guizhou Academy of Sciences, Guiyang, Guizhou, China.
PLoS One. 2023 Feb 21;18(2):e0281854. doi: 10.1371/journal.pone.0281854. eCollection 2023.
Passion fruit (Passiflora edulis) is widely grown in tropical and subtropical regions, showing high economic and ornamental value. Microorganisms are indicators for the stability and health of the soil ecosystem, which can affect the yield and quality of passion fruit under continuous cropping. High-throughput sequencing and interactive analysis were used to analyse the variation of microbial communities in the noncultivated soil (NCS), cultivated soil (CS), and the rhizosphere soil of purple passion fruit (Passiflora edulis f. edulis ×Passiflora edulis f. flavicarpa, RP) and yellow passion fruit (Passiflora edulis f. flavicarpa, RY). An average of 98,001 high-quality fungal internal transcribed spacer (ITS) sequences, mainly from Ascomycota, Basidiomycota, Mortierellomycota, Mucoromycota and Glomeromycota, as well as an average of 71,299 high-quality bacterial 16S rRNA sequences, mainly from Proteobacteria, Actinobacteria, Acidobacteria, Firmicutes and Chloroflexi, were obtained per sample. It was found that the continuous cropping of passion fruit increased the richness but reduced the diversity of soil fungi, while it dramatically increased the richness and diversity of soil bacteria. In addition, during the continuous cropping, grafting different scions in the same rootstock contributed to the aggregation of differential rhizosphere microbial communities. Among fungal genera, Trichoderma showed higher abundance in RY than in RP and CS, while the opposite was observed in the pathogen Fusarium. Moreover, the co-occurrence network and potential function analyses also showed that the appearance of Trichoderma was related to Fusarium and its contribution to plant metabolism was significantly greater in RY than in RP and CS. In conclusion, the rhizosphere of yellow passion fruit may be beneficial for the enrichment of disease-resistant microbes, such as Trichoderma, which may be an important factor inducing stronger resistance to stem rot. It will help to form a potential strategy for overcoming the pathogen-mediated obstacles in passion fruit and improve its yield and quality.
百香果（Passiflora edulis）广泛种植于热带和亚热带地区，具有很高的经济和观赏价值。微生物是土壤生态系统稳定性和健康的指标，它们会影响百香果在连作下的产量和品质。本研究采用高通量测序和交互分析的方法，研究了在非种植土壤（NCS）、种植土壤（CS）以及紫色百香果（Passiflora edulis f. edulis×Passiflora edulis f. flavicarpa，RP）和黄色百香果（Passiflora edulis f. flavicarpa，RY）根际土壤中微生物群落的变化。每个样本平均获得了 98001 条高质量的真菌内部转录间隔区（ITS）序列，主要来自子囊菌门、担子菌门、毛霉门、Mucoromycota 和 Glomeromycota，以及 71299 条高质量的细菌 16S rRNA 序列，主要来自变形菌门、放线菌门、酸杆菌门、厚壁菌门和绿弯菌门。研究发现，百香果连作增加了土壤真菌的丰富度，但降低了其多样性，而土壤细菌的丰富度和多样性则显著增加。此外，在连作过程中，同一砧木上嫁接不同的接穗有助于聚集差异的根际微生物群落。在真菌属中， Trichoderma 在 RY 中的丰度高于 RP 和 CS，而病原菌 Fusarium 的情况则相反。此外，共现网络和潜在功能分析也表明， Trichoderma 的出现与 Fusarium 有关，其对植物代谢的贡献在 RY 中明显大于 RP 和 CS。综上所述，黄色百香果的根际可能有利于富集抗病微生物，如 Trichoderma，这可能是诱导其对茎腐病更强抗性的一个重要因素。这将有助于形成一种克服百香果中病原菌介导障碍、提高其产量和品质的潜在策略。
