Leiter E H, Coleman D L, Eppig J J
In Vitro. 1979 Jul;15(7):507-21. doi: 10.1007/BF02618153.
Ultrastructural characteristics as well as secretory and biosynthetic behavior of monolayer pancreatic cell cultures established from 4-day-old C57BL/KsJ misty diabetic (m db/m db) mice have been studied in comparison to normal littermate controls. Hypersecretion of glucagon by alpha-cells from BL/Ks misty diabetic mice after 2 days in vitro was found to precede any hyperfunction of the insulin-secreting beta-cells. The increased level of glucagon-release in BL/Ks cell cultures from diabetic mice was accompanied by a greatly enhanced level of incorporation of [3H]tryptophan into glucagon-like molecules whose specific radioactivity was up to 15-fold higher than that observed in cultures from genetic controls. The finding of an alpha-cell dysfunction in cultures established from preweaning diabetic BL/Ks mice suggests that glucagon could play an early role in shaping the events that culminate in the expression of frank diabetes in this inbred strain.
已对从4日龄C57BL/KsJ雾状糖尿病(m db/m db)小鼠建立的单层胰腺细胞培养物的超微结构特征以及分泌和生物合成行为进行了研究,并与正常同窝对照进行了比较。发现来自BL/Ks雾状糖尿病小鼠的α细胞在体外培养2天后胰高血糖素分泌过多,这先于胰岛素分泌β细胞的任何功能亢进。糖尿病小鼠的BL/Ks细胞培养物中胰高血糖素释放水平的增加伴随着[3H]色氨酸掺入胰高血糖素样分子的水平大大提高,其比放射性比基因对照培养物中观察到的高15倍。在断奶前糖尿病BL/Ks小鼠建立的培养物中发现α细胞功能障碍,这表明胰高血糖素可能在塑造导致该近交系显性糖尿病表达的事件中起早期作用。