School of Public Health, Chongqing Medical University, Chongqing 400016, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing School, University of Chinese Academy of Sciences, Chongqing 400714, China; University of Chinese Academy of Sciences, Beijing 100049, China.
School of Public Health, Chongqing Medical University, Chongqing 400016, China.
J Hazard Mater. 2024 Dec 5;480:136030. doi: 10.1016/j.jhazmat.2024.136030. Epub 2024 Oct 1.
Rising glbal population and plastic consumption have caused a dramatic increase in plastic waste, leading to micro- and nanoplastic ingestion by aquatic organisms and subsequent bioaccumulation in their tissues. This transfer to higher trophic levels raises nanoplastic concentrations and bioavailability, potentially harming organisms' health and development. This poses a risk to human health via seafood. To address these issues, this study assesses the toxicological impacts of nanoplastics (NPs) on brine shrimp (Artemia franciscana) and their trophic transfer to zebrafish. The research unveiled concentration-dependent bioaccumulation of NPs in zebrafish and Artemia franciscana (A. franciscana). Polystyrene nanoplastics (PS-NPs) exhibited higher accumulation in A. franciscana whereas PP-NPs showed greater accumulation in zebrafish gut. Histopathological analysis under PS-NPs exposure revealed significant tissue alterations, indicative of inflammatory responses and impaired mucosal barrier integrity. Gene expression analyses confirmed these findings, showing activation of the P38-MAPK pathway by PS-NPs, which correlated with increased inflammatory cytokines. Additionally, PE-NPs activated the JNK-MAPK pathway, while PP-NPs exposure triggered the NOD-like receptor signaling pathway. Moreover, the composition of gut microbiota shifted to a dysbiotic state, characterized by an increase in pathogenic bacteria in the PS-NPs and PP-NPs groups, elevating the risk of developing Inflammatory Bowel Disease (IBD). PS-NPs were regarded as the most toxic due to their lower stability and higher aggregation tendencies, followed by PP-NPs and PE-NPs. Taken together, the overall study highlighted the complex interactions between NPs, gut microbiota, and host health, emphasizing the importance of thoroughly assessing the ecological and physiological impacts of nanoplastic pollution.
全球人口增长和塑料消费的增加导致了塑料垃圾的急剧增加，使水生生物摄入微塑料和纳米塑料，并在其组织中随后发生生物蓄积。这种转移到更高的营养级会增加纳米塑料的浓度和生物利用度，可能会对生物的健康和发育造成危害。这种通过海鲜对人类健康构成风险。为了解决这些问题，本研究评估了纳米塑料（NPs）对卤虫（Artemia franciscana）的毒理学影响及其对斑马鱼的营养传递。研究揭示了 NPs 在斑马鱼和卤虫（A. franciscana）中的浓度依赖性生物蓄积。聚苯乙烯纳米塑料（PS-NPs）在 A. franciscana 中的积累较高，而聚丙烯纳米塑料（PP-NPs）在斑马鱼肠道中的积累较高。PS-NPs 暴露下的组织病理学分析显示出显著的组织改变，表明存在炎症反应和粘膜屏障完整性受损。基因表达分析证实了这些发现，表明 PS-NPs 激活了 P38-MAPK 途径，这与炎症细胞因子的增加有关。此外，PE-NPs 激活了 JNK-MAPK 途径，而 PP-NPs 暴露则触发了 NOD 样受体信号通路。此外，肠道微生物群的组成发生了紊乱，表现为 PS-NPs 和 PP-NPs 组中致病菌的增加，增加了患炎症性肠病（IBD）的风险。由于 PS-NPs 的稳定性较低且更容易聚集，因此被认为是最有毒的，其次是 PP-NPs 和 PE-NPs。总的来说，这项研究强调了 NPs、肠道微生物群和宿主健康之间的复杂相互作用，强调了彻底评估纳米塑料污染对生态和生理影响的重要性。
