Jansen A M, Moriearty P L, Castro B G, Deane M P
Trans R Soc Trop Med Hyg. 1985;79(4):474-7. doi: 10.1016/0035-9203(85)90069-0.
The use of an indirect fluorescent antibody test (IFAT) performed in a "sandwich" technique has demonstrated: (i) the usefulness of the test for the diagnosis of Trypanosoma cruzi infection in the opossum Didelphis marsupialis; (ii) the existence of differences in the serological response of the opossum, that were related to the parasite strain and were clearly evident during the follow-up of experimental infections in laboratory born specimens; (iii) that, despite a good correlation between serological and parasitological examinations, IFAT was the most sensitive diagnostic test used, followed by xenodiagnosis; and, (iv) that in general, the opossum D. marsupialis seems to be a good responder to T. cruzi antigens.
采用“夹心”技术进行间接荧光抗体试验（IFAT）已证明：（i）该试验对诊断负鼠（Didelphis marsupialis）感染克氏锥虫有用；（ii）负鼠的血清学反应存在差异，这些差异与寄生虫菌株有关，并且在实验室出生标本的实验性感染随访期间明显可见；（iii）尽管血清学和寄生虫学检查之间有良好的相关性，但IFAT是最敏感的诊断试验，其次是异种诊断；以及（iv）总体而言，负鼠（D. marsupialis）似乎对克氏锥虫抗原反应良好。
