State Key Laboratory of Rice Biology, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310000, Zhejiang Province, China.
Hangzhou Ruifeng Biosciences Co., Ltd., 1500 Wenyi Road, Building 1, Room103, Hangzhou 310000, Zhejiang Province, China.
Int J Biol Macromol. 2024 Nov;281(Pt 1):136219. doi: 10.1016/j.ijbiomac.2024.136219. Epub 2024 Oct 2.
Lepidopteran pests frequently cause significant damage to Sunflowers (Helianthus annuus). In this study, the insect resistant fusion gene Cry1Ab-Vip3Af2 was transformed into sunflower by Agrobacterium-mediated transformation. A transgenic event, named MCPN-7, was selected and characterized for its high resistance to both yellow peach moth (Dichocrocis punctiferalis) and cotton bollworm (Helicoverpa armigera), two polyphagous pests feeding on various plants including sunflower. The neonates of both species feeding on MCPN-7 resulted to 100 % mortality within 72 h in laboratory bioassays. No significant damage caused by the two insects was observed in field trials of MCPN-7. ELISA analysis revealed that the fusion protein was predominantly expressed in leaves, seeds and heads. The flanking genomic sequence of the T-DNA of the event MCPN-7 was determined and confirmed by PCR analysis. In conclusion, the transgenic sunflowers obtained in this study is highly resistant to wide spectrum of Lepidopteran insect pests and could potentially be a candidate event for commercial development.
鳞翅目害虫经常对向日葵（Helianthus annuus）造成严重损害。在这项研究中，通过农杆菌介导的转化将抗虫融合基因 Cry1Ab-Vip3Af2 转化到向日葵中。选择了一个名为 MCPN-7 的转基因事件，该事件对两种多食性害虫，即黄桃蛾（Dichocrocis punctiferalis）和棉铃虫（Helicoverpa armigera）具有高抗性，这两种害虫以包括向日葵在内的各种植物为食。在实验室生物测定中，两种昆虫的幼虫在食用 MCPN-7 后 72 小时内全部死亡。在 MCPN-7 的田间试验中，没有观察到这两种昆虫造成的明显损害。ELISA 分析表明，融合蛋白主要在叶片、种子和头部表达。通过 PCR 分析确定了事件 MCPN-7 的 T-DNA 侧翼基因组序列，并得到了确认。总之，本研究获得的转基因向日葵对广泛的鳞翅目昆虫具有高度抗性，可能成为商业开发的候选事件。
