Xie Ning, Ma Tengfei, Gao Yuan, Fu Jiani, Tan Chune, Wang Zheng, Wang Zhuo, Krüger-Haker Henrike, Feßler Andrea T, Wu Congming, Shen Jianzhong, Schwarz Stefan, Wang Yang
Technology Innovation Center for Food Safety Surveillance and Detection (Hainan), Sanya Institute of China Agricultural University, Sanya, Hainan, China; National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Vito Diagnostic Technology Company Limited, Hangzhou, Zhejiang, China.
J Glob Antimicrob Resist. 2024 Dec;39:170-174. doi: 10.1016/j.jgar.2024.09.004. Epub 2024 Oct 1.
The emergence and transmission of the multidrug resistance gene cfr have raised public health concerns worldwide.
Multidrug-resistant Staphylococcus equorum isolates can pose a threat to public health. In this study, we have characterised the whole-genome of one Staphylococcus equorum isolate harbouring two distinct cfr-carrying plasmids.
Antimicrobial susceptibility testing was performed by broth microdilution. Genomic DNA was sequenced using both the Illumina HiSeq X Ten and Nanopore MinION platforms. De novo hybrid assembly was performed by Unicycler. Genomic data were assessed by in silico prediction and bioinformatic tools.
Staphylococcus equorum isolate SN42 exhibited resistance or high MICs to linezolid, erythromycin, tetracycline, oxacillin, clindamycin, virginiamycin, tiamulin, chloramphenicol and florfenicol. It carried two cfr-harbouring plasmids: the RepA N-family plasmid pSN42-51 K and the Inc18-family plasmid pSN42-50 K. These two plasmids exhibited low structural similarities to the so far reported cfr-carrying plasmids. Both plasmids harboured an arsenic resistance operon, copper and cadmium resistance genes as well as the lincosamide-pleuromutilin-streptogramin A resistance gene lsa(B). In addition, plasmid pSN42-51 K carried two erm(B) genes for macrolide-lincosamide-streptogramin B resistance, the streptomycin resistance gene ant(6)-Ia as well as mercury resistance genes while pSN42-50 K was associated with the heavy metal translocating P-type ATPase gene hmtp. The co-carriage and co-existence of these antimicrobial resistance and heavy metal resistance genes increases the likelihood of co-selection of the cfr-carrying plasmids.
This is the first report of S. equorum carrying two distinct cfr-carrying plasmids, underscoring the need for ongoing surveillance to address the potential dissemination of multi-drug resistance in bacteria from food-producing animals to ensure food safety and public health.
多重耐药基因cfr的出现和传播已引起全球范围内的公共卫生关注。
多重耐药的马胃葡萄球菌分离株可能对公共卫生构成威胁。在本研究中，我们对一株携带两个不同的携带cfr质粒的马胃葡萄球菌分离株进行了全基因组特征分析。
采用肉汤微量稀释法进行药敏试验。使用Illumina HiSeq X Ten和Nanopore MinION平台对基因组DNA进行测序。通过Unicycler进行从头混合组装。利用计算机预测和生物信息学工具评估基因组数据。
马胃葡萄球菌分离株SN42对利奈唑胺、红霉素、四环素、苯唑西林、克林霉素、维吉尼亚霉素、替米考星、氯霉素和氟苯尼考表现出耐药性或高最低抑菌浓度（MIC）。它携带两个携带cfr的质粒：RepA N家族质粒pSN42-51 K和Inc18家族质粒pSN42-50 K。这两个质粒与迄今报道的携带cfr的质粒结构相似性较低。两个质粒都含有一个抗砷操纵子、铜和镉抗性基因以及林可酰胺-截短侧耳素-链阳菌素A抗性基因lsa(B)。此外，质粒pSN42-51 K携带两个用于大环内酯-林可酰胺-链阳菌素B抗性的erm(B)基因、链霉素抗性基因ant(6)-Ia以及汞抗性基因，而pSN42-50 K与重金属转运P型ATP酶基因hmtp相关联。这些抗菌抗性和重金属抗性基因的共同携带和共存增加了携带cfr质粒共同选择的可能性。
这是关于马胃葡萄球菌携带两个不同的携带cfr质粒的首次报道，强调了持续监测以应对多药耐药性从产食动物细菌向潜在传播的必要性，以确保食品安全和公共卫生。
