Food Sciences Department, Federal University of Lavras (UFLA), Lavras, Minas Gerais, 37200-900MG, Brazil.
Chemistry Department, Federal University of Lavras (UFLA), Lavras, Minas Gerais, 37200-900MG, Brazil.
FEMS Microbiol Lett. 2024 Jan 9;371. doi: 10.1093/femsle/fnae081.
The antifungal and antimycotoxigenic activities of the essential oils (EO) from Cuminum cyminum and Laurus nobilis, and their respective principal compounds, cuminaldehyde and 1,8-cineole, were evaluated against fungi of the genus Aspergillus: A. carbonarius, A. niger, A. ochraceus, and A. westerdijkiae. The antifungal activity was determined by the contact method and the mycelial growth of the fungi was evaluated. Scanning electron microscopic (SEM) images were obtained to suggest modes of action of the compounds analysed. The antimycotoxigenic activity was determined by high-performance liquid chromatograph. Aspergillus carbonarius was completely inhibited by cumin EO (500 µl l-1), by laurel EO and by cuminaldehyde (5000 µl l-1). The cumin EO (500 µl l-1) completely inhibited the growth of A. niger. All the samples inhibited the mycelial growth of A. ochraceus, especially cumin EO and cuminaldehyde (250 µl l-1). Aspergillus westerdijkiae was completely inhibited by cumin EO and cuminaldehyde (1000 µl l-1), by laurel EO and 1,8-cineole (10 000 µl l-1). A decrease in the production of ochratoxin A (OTA) was observed post-treatment, except in A. ochraceus, only inhibited by laurel EO. SEM images showed morphological changes in fungal structures and spore inhibition post-treatment. The results confirmed the antifungal and antimycotoxigenic effect of EO and their principal constituents on fungi evaluated.
研究了芫荽和月桂精油及其主要化合物肉桂醛和 1,8-桉叶素对黑曲霉、黑曲霉、棕曲霉和韦斯狄克曲霉属真菌的抗真菌和抗真菌毒素活性。采用接触法测定了精油的抗真菌活性，评价了真菌的菌丝生长情况。获得了扫描电子显微镜（SEM）图像，以提出分析化合物的作用模式。采用高效液相色谱法测定了抗真菌毒素活性。芫荽精油（500 μl l-1）、月桂精油和肉桂醛（5000 μl l-1）完全抑制了黑曲霉的生长。芫荽精油（500 μl l-1）完全抑制了黑曲霉的生长。所有样品均抑制了棕曲霉的菌丝生长，尤其是芫荽精油和肉桂醛（250 μl l-1）。芫荽精油和肉桂醛（1000 μl l-1）、月桂精油和 1,8-桉叶素（10000 μl l-1）完全抑制了韦斯狄克曲霉的生长。除了仅被月桂精油抑制的棕曲霉外，处理后观察到赭曲霉毒素 A（OTA）产量下降。SEM 图像显示了处理后真菌结构和孢子抑制的形态变化。结果证实了精油及其主要成分对评价真菌的抗真菌和抗真菌毒素作用。
