Koley Suprotim, Risla Sherin Paacha Kandy, Nayak Minati, Barooah Nilotpal, Bhasikuttan Achikanath C, Mohanty Jyotirmayee
Radiation & Photochemistry Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
Homi Bhabha National Institute, Anushaktinagar, Mumbai 400094, India.
ACS Phys Chem Au. 2024 Jul 22;4(5):522-530. doi: 10.1021/acsphyschemau.4c00027. eCollection 2024 Sep 25.
Surface-functionalized noble metal nanoparticles with macrocyclic hosts have attracted enormous research interest owing to their applications in drug delivery, catalysis, bioimaging, etc. Stable -sulfonatocalix[6]arene-functionalized gold nanoparticles (SCx6AuNPs) of the sizes ∼7.5 nm have been synthesized and characterized by using UV-vis absorption, transmission electron microscopy, and surface-enhanced Raman spectroscopy measurements. The efficient uptake and stimuli-responsive release of doxorubicin (Dox), an anticancer drug, by the SCx6AuNPs have been established for targeted drug delivery application. The decreased cytotoxicity of Dox loaded on SCx6AuNPs, especially toward normal cell lines, and its multistimuli responsive release validated in both cancerous (A549) and normal (W126) cell lines find promising for selectively targeted drug delivery applications toward cancer cells. At the cellular level, this study also establishes the efficient uptake of the SCx6AuNP nanoconjugates, and its validation has been done by bioimaging measurement by using thioflavin T (ThT) dye loaded on to SCx6AuNPs instead of Dox as the fluorescent tracking probe. The bright fluorescence microscopic image of ThT-SCx6AuNP-stained cancerous cell lines corroborates the uptake of SCx6AuNPs by the cell lines and its projected utility for drug delivery and bioimaging applications.
带有大环主体的表面功能化贵金属纳米颗粒因其在药物递送、催化、生物成像等方面的应用而引起了极大的研究兴趣。已合成了尺寸约为7.5 nm的稳定的磺化杯[6]芳烃功能化金纳米颗粒（SCx6AuNPs），并通过紫外可见吸收、透射电子显微镜和表面增强拉曼光谱测量对其进行了表征。已证实SCx6AuNPs对抗癌药物阿霉素（Dox）具有高效摄取和刺激响应释放特性，可用于靶向药物递送应用。负载在SCx6AuNPs上的Dox细胞毒性降低，尤其是对正常细胞系，并且其在癌细胞系（A549）和正常细胞系（W126）中均得到验证的多刺激响应释放特性，在针对癌细胞的选择性靶向药物递送应用中前景广阔。在细胞水平上，本研究还证实了SCx6AuNP纳米缀合物的高效摄取，并通过使用负载在SCx6AuNPs上的硫黄素T（ThT）染料而非Dox作为荧光追踪探针进行生物成像测量来进行验证。ThT-SCx6AuNP染色的癌细胞系的明亮荧光显微镜图像证实了细胞系对SCx6AuNPs的摄取及其在药物递送和生物成像应用中的预期效用。
