Yong Zi-Ling, Chen Yu-Ting, Chan Ching, Lee Guan-Chiun
School of Life Science, National Taiwan Normal University, Taipei 11677, Taiwan.
Institute of Genomics and Bioinformatics, National Chung Hsing University, Taichung 402, Taiwan.
J Agric Food Chem. 2024 Oct 4. doi: 10.1021/acs.jafc.4c07364.
Trehalose, a versatile disaccharide renowned for its unique physical and chemical properties, finds extensive application in the food, pharmaceutical, and cosmetic industries. While conventional extraction methods face challenges, enzymatic conversion offers a promising avenue for the industrial production of trehalose. This study delves into a novel synthetic approach utilizing a recombinant enzyme, merging the thermostable trehalose synthase domain from with a cellulose binding domain. Immobilization of this enzyme on cellulose matrices enhances stability and facilitates product purification, opening avenues for efficient enzymatic synthesis. Notably, the engineered enzyme demonstrates additional activity, converting sucrose into trehalulose. This dual functionality, combined with immobilization strategies, holds immense potential for scalable and cost-effective production of trehalose and trehalulose, offering promising prospects in various industrial and biomedical applications.
海藻糖是一种因其独特的物理和化学性质而闻名的多功能二糖，在食品、制药和化妆品行业有着广泛的应用。虽然传统的提取方法面临挑战，但酶促转化为海藻糖的工业化生产提供了一条有前景的途径。本研究深入探讨了一种利用重组酶的新型合成方法，该方法将来自[具体来源未提及]的耐热海藻糖合酶结构域与纤维素结合结构域融合。将这种酶固定在纤维素基质上可提高稳定性并便于产品纯化，为高效酶促合成开辟了道路。值得注意的是，工程化酶表现出额外的活性，可将蔗糖转化为海藻酮糖。这种双重功能与固定化策略相结合，在海藻糖和海藻酮糖的可扩展且具有成本效益的生产方面具有巨大潜力，在各种工业和生物医学应用中展现出广阔前景。
