Sato Masato, Tokodai Kazuaki, Okada Kaoru, Ogasawara Hiroyuki, Tanaka Miyako, Hoshiai Tetsuro, Saito Masatoshi, Sugawara Hirofumi, Akamatsu Daijirou, Unno Michiaki, Goto Masafumi, Kamei Takashi
Department of Surgery, Tohoku University, Sendai, Japan.
Department of Gynecology and Obstetrics, Tohoku University, Sendai, Japan.
J Biomater Appl. 2025 Feb;39(7):789-796. doi: 10.1177/08853282241289919. Epub 2024 Oct 4.
Although the human amniotic membrane (hAM) has been demonstrated to promote angiogenesis, its efficacy in healing ischemic wounds remains unknown. Therefore, the current study aimed to evaluate the potential of hAM as a dressing for treating ischemic wounds. The inferior abdominal wall arteries and veins of male rats were divided, and an ischemic wound was created on each side of the abdominal wall. Of the two ischemic wounds created, only one was covered with hAM, and its wound healing effect was determined by measuring the wound area. Angiogenesis was assessed by measuring microvessel density (MVD). On day 5, the mean wound area changed from 400 mm to 335.4 (260-450) mm in the hAM group and to 459 (306-570) mm in the control group ( = 0.0051). MVD was 19.0 (10.4-24.6) in the hAM group and 15.1 (10.6-20.8) in the control group ( = 0.0026). No significant differences in local pro- and anti-inflammatory cytokine levels were observed between the two groups. Histological examination revealed no rejection of the transplanted hAM. Therefore, the hAM may serve as a novel wound dressing that can promote angiogenesis and healing in ischemic wounds.
尽管人羊膜（hAM）已被证明可促进血管生成，但其在愈合缺血性伤口方面的疗效仍不清楚。因此，本研究旨在评估hAM作为治疗缺血性伤口敷料的潜力。将雄性大鼠的下腹壁动静脉分开，并在腹壁两侧制造缺血性伤口。在制造的两个缺血性伤口中，只有一个用hAM覆盖，并通过测量伤口面积来确定其伤口愈合效果。通过测量微血管密度（MVD）评估血管生成。在第5天，hAM组的平均伤口面积从400平方毫米变为335.4（260 - 450）平方毫米，对照组变为459（306 - 570）平方毫米（P = 0.0051）。hAM组的MVD为19.0（1起，0.4 - 24.6），对照组为15.1（10.6 - 20.8）（P = 0.0026）。两组之间局部促炎和抗炎细胞因子水平未观察到显著差异。组织学检查显示未出现对移植hAM的排斥反应。因此，hAM可作为一种新型伤口敷料，促进缺血性伤口的血管生成和愈合。 （注：原文中“1起”疑似有误，未在翻译中体现该错误内容）
