Christodoulou Michael S, Pinna Cecilia, Ghosh Sharmila, Princiotto Salvatore, Sacchi Francesca, Brunetti Barbara, Pizzatti Cristina, Musso Loana, Cortesi Paolo, Pinto Andrea, Kunova Andrea, Dallavalle Sabrina
Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Via Celoria 2, 20133, Milan 20133, Italy.
J Agric Food Chem. 2024 Oct 4. doi: 10.1021/acs.jafc.4c02909.
The rice-blast fungus poses a significant threat to rice production worldwide. Ferroptosis, an iron-dependent form of regulated cell death, has recently been reported to be involved in pathogenicity during plant-fungal interactions. Ferroptosis regulates the developmental cell death of conidia necessary for appressorium maturation. In this study, we have established that a series of benzamides containing a chelating catechol moiety suppresses the formation/maturation of appressoria, which are essential for host infection by the rice blast fungus. Moreover, for the most active compounds we have shown that their activity can be at least partially reversed by adding exogenous Fe. These results highlight the close association between iron availability and appressorium maturation, opening new avenues for the development of targeted strategies for management.
稻瘟病菌对全球水稻生产构成重大威胁。铁死亡是一种铁依赖性的程序性细胞死亡形式，最近有报道称其参与植物与真菌相互作用过程中的致病性。铁死亡调节附着胞成熟所需的分生孢子的发育性细胞死亡。在本研究中，我们确定了一系列含有螯合儿茶酚部分的苯甲酰胺可抑制附着胞的形成/成熟，而附着胞是稻瘟病菌感染宿主所必需的。此外，对于最具活性的化合物，我们发现通过添加外源铁，其活性至少可以部分逆转。这些结果突出了铁的可利用性与附着胞成熟之间的紧密联系，为开发针对性的防治策略开辟了新途径。
