功能化修饰的氧化石墨烯作为一种替代纳米载体，用于增强双链RNA递送以改善基于RNA干扰的害虫防治

Functionally Modified Graphene Oxide as an Alternative Nanovehicle for Enhanced dsRNA Delivery in Improving RNAi-Based Insect Pest Control.

作者信息

Xue Qi, Li Jiangjie, Vereecken Sven, Li Qiqiong, Zhi Zijian, Dubruel Peter, Taning Clauvis Nji Tizi, De Schutter Kristof

机构信息

Molecular Entomology Laboratory, Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent 9000, Belgium.

Polymer Chemistry and Biomaterials group, Centre of Macromolecular Chemistry, Department of Organic and Macromolecular Chemistry, Faculty of Sciences, Ghent University, Ghent 9000, Belgium.

出版信息

J Agric Food Chem. 2024 Oct 4. doi: 10.1021/acs.jafc.4c05215.

DOI:10.1021/acs.jafc.4c05215

PMID:39365919

Abstract

RNA interference (RNAi) has shown substantial promise as a sustainable pest management solution. However, the efficacy of RNAi-based insecticides heavily relies on advanced nanocarrier-mediated delivery systems. In this study, we modified raw graphene oxide into positively charged nanocarriers (GONs) tailored to bind with double-stranded RNA (dsRNA). The resulting GONs@dsRNA complexes demonstrated a small particle size (106 nm) and maintained stability under various conditions, including insect gut extracts, extreme pH, and extreme temperature. Furthermore, GONs efficiently transported dsRNA molecules into Drosophila S2 cells and Lepidoptera Sf9 cells, leading to an enhanced target transcript knockdown. Targeting the vacuolar ATPase gene, , induced significant mortality and target transcript knockdown in adults but not in . Finally, GONs@dsRNA complexes exhibited negligible cytotoxicity at both the cellular and organismal levels. This study demonstrates the potential of GONs as a biosafe nanovehicle for efficient dsRNA delivery into insects, presenting an alternative strategy for advancing RNAi applications in fundamental studies and pest control.

摘要

RNA干扰（RNAi）作为一种可持续的害虫管理解决方案已显示出巨大的前景。然而，基于RNAi的杀虫剂的功效在很大程度上依赖于先进的纳米载体介导的递送系统。在本研究中，我们将原始氧化石墨烯修饰为带正电荷的纳米载体（GONs），专门用于与双链RNA（dsRNA）结合。所得的GONs@dsRNA复合物显示出小粒径（106纳米），并在包括昆虫肠道提取物、极端pH值和极端温度在内的各种条件下保持稳定。此外，GONs能有效地将dsRNA分子转运到果蝇S2细胞和鳞翅目Sf9细胞中，导致靶转录本的敲低增强。靶向液泡ATP酶基因，在成虫中诱导了显著的死亡率和靶转录本敲低，但在中未出现。最后，GONs@dsRNA复合物在细胞和生物水平上均表现出可忽略不计的细胞毒性。本研究证明了GONs作为一种生物安全的纳米载体，可将dsRNA高效递送至昆虫体内的潜力，为推进RNAi在基础研究和害虫防治中的应用提供了一种替代策略。