一种从小鼠FM3A细胞中分离突变体突变株及其特性鉴定的改进方法。
An improved method for isolation of mutator mutants from mouse FM3A cells and their characterization.
作者信息
Hyodo M, Ito N, Yamashita M, Suzuki K
出版信息
Cell Struct Funct. 1985 Dec;10(4):383-90. doi: 10.1247/csf.10.383.
An improved method to select mutator mutants was developed. By this new method, mutator mutants were isolated efficiently, and 7 mutants were obtained from cultured mouse FM3A cells. These mutator mutants have an elevated rate of spontaneous mutation at 3 genetic loci (resistance to ouabain, blasticidin S, and tunicamycin). The sensitivity of these mutants to aphidicolin and arabinofuranosylcytosine was the same as in the wild-type cells. Determination of the size of the cellular dNTP pool revealed that there was no large imbalance in the precursor pool in the mutator mutants. These results suggested that the mutator character may be due to alteration in some factor(s) correlated directly to DNA replication. Also, there was no change in the sensitivity of all these mutator mutants to DNA damaging agents.
开发了一种改进的选择突变体的方法。通过这种新方法，有效地分离出了突变体，并且从培养的小鼠FM3A细胞中获得了7个突变体。这些突变体在3个基因位点（对哇巴因、杀稻瘟菌素S和衣霉素的抗性）的自发突变率有所提高。这些突变体对阿非科林和阿糖胞苷的敏感性与野生型细胞相同。对细胞dNTP库大小的测定表明，突变体中前体库没有大的失衡。这些结果表明，突变体特性可能是由于与DNA复制直接相关的某些因素发生了改变。此外，所有这些突变体对DNA损伤剂的敏感性没有变化。
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