State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, PR China.
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, PR China.
Phytochemistry. 2025 Jan;229:114296. doi: 10.1016/j.phytochem.2024.114296. Epub 2024 Oct 2.
Plant-derived natural products remain crucial in drug development. However, the identification of undescribed natural products is becoming increasingly challenging. A comprehensive strategy combining LC-MS with diagnostic ions was proposed for the discovery of undescribed 5-methylcoumarin meroterpenoids. Thirteen undescribed 5-methylcoumarin meroterpenoids, including five pairs of enantiomers (1a/1b and 5a/5b-8a/8b), were isolated from the whole plant of Gerbera piloselloides. Their structures and absolute configurations were unambiguously determined based on their spectroscopic data, calculated and experimental ECD data and X-ray diffraction analysis. Bioassays conducted on scopolamine-induced injury PC12 cells revealed that compounds 5a/5b, 7a/7b and 8a/8b possessed mild protective effects. Additionally, compounds 2 and 8 showed notable IL-6 inhibition in lipopolysaccharide-induced BEAS-2B cells.
植物源天然产物仍然是药物开发的关键。然而，描述未知天然产物的难度越来越大。本文提出了一种将 LC-MS 与诊断离子相结合的综合策略，用于发现未知的 5-甲基香豆素倍半萜类化合物。从毛叶金光菊全草中分离得到了 13 个未知的 5-甲基香豆素倍半萜类化合物，包括 5 对非对映异构体（1a/1b 和 5a/5b-8a/8b）。基于其光谱数据、计算和实验 ECD 数据以及 X 射线衍射分析，明确确定了它们的结构和绝对构型。在东莨菪碱诱导损伤的 PC12 细胞上进行的生物测定表明，化合物 5a/5b、7a/7b 和 8a/8b 具有轻微的保护作用。此外，化合物 2 和 8 对脂多糖诱导的 BEAS-2B 细胞中 IL-6 的抑制作用显著。
