Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region, Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080, China.
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region, Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080, China.
Int J Biol Macromol. 2024 Nov;281(Pt 1):136270. doi: 10.1016/j.ijbiomac.2024.136270. Epub 2024 Oct 2.
Multifunctional food packaging composite films were prepared using Pediococcus acidilactici J1 exopolysaccharide (EPS), potato starch (PS) and TiO nanoparticles by casting method. The microstructure, physicochemical properties and antibacterial activity of EPS/PS composite films with different weight ratio of TiO nanoparticles were characterized. Transmission electron microscope (TEM), scanning electron microscopy (SEM) and atomic force microscopy (AFM) revealed the uniform distribution of TiO nanoparticles in the EPS/PS matrix. Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) results indicated that the interaction between polymers and nanoparticles through non-covalent bonds. When TiO nanoparticles were added at 1 % (wt), the composite film had higher barrier properties against water vapor and UV-vis light, and better mechanical properties then EPS/PS film. Notably, EPS/PS/1%TiO composite film exhibited good antioxidant and antibacterial activity against Escherichia coli and Staphylococcus aureus. Through the analysis of the quality indexes and microbial community structure during the storage of chilled meat, the composite film slowed the oxidation rate of chilled meat and inhibited the growth of dominant spoilage bacteria, effectively extending its shelf life. All results suggested that EPS/PS/1%TiO composite film could serve as an effective packaging material for chilled meat, providing a novel approach to solve its limited shelf-life problem.
采用聚乳酸酸乳杆菌 J1 胞外多糖（EPS）、马铃薯淀粉（PS）和 TiO 纳米粒子通过浇铸法制备了多功能食品包装复合膜。通过透射电子显微镜（TEM）、扫描电子显微镜（SEM）和原子力显微镜（AFM）研究了不同比例 TiO 纳米粒子的 EPS/PS 复合膜的微观结构、物理化学性质和抗菌活性。结果表明，TiO 纳米粒子在 EPS/PS 基质中均匀分布。傅里叶变换红外光谱（FT-IR）和 X 射线衍射（XRD）结果表明，聚合物和纳米粒子通过非共价键相互作用。当 TiO 纳米粒子添加量为 1%（wt）时，复合膜对水蒸气和紫外可见光具有更高的阻隔性能，且力学性能优于 EPS/PS 膜。值得注意的是，EPS/PS/1%TiO 复合膜对大肠杆菌和金黄色葡萄球菌表现出良好的抗氧化和抗菌活性。通过对冷藏肉贮藏过程中质量指标和微生物群落结构的分析，复合膜减缓了冷藏肉的氧化速度，抑制了优势腐败菌的生长，有效延长了其货架期。所有结果表明，EPS/PS/1%TiO 复合膜可用作冷藏肉的有效包装材料，为解决其货架期有限的问题提供了一种新方法。
