JNU-UPM International Joint Laboratory on Plant Oil Processing and Safety, Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.
School of Science, Monash University Malaysia, Bandar Sunway 47500, Selangor, Malaysia.
Int J Biol Macromol. 2024 Nov;281(Pt 1):136223. doi: 10.1016/j.ijbiomac.2024.136223. Epub 2024 Oct 2.
Diacylglycerol-based nanoparticles are promising bioactive delivery systems. However, limited understanding of their interaction with biological entities restricts their clinical use. This study investigated the protein corona formed on medium and long chain diacylglycerol (MLCD)-based solid lipid nanoparticles (NPs) modified by Polyoxethylene stearate (PEG) and compared to glyceryl tristearate (TG) and cetyl palmitate (CP) nanoparticles. Bovine serum albumin (BSA) formed corona with MLCD NPs through hydrophobic interactions and hydrogen bonding, contributing to a decrease in α-helix, an increase in β-sheet and a change in the microenvironment of Tyr residues. Owing to higher lipid hydrophilicity, MLCD NPs showed a much lower affinity for BSA than TG and CP NPs, and the binding constant with BSA was increased for larger NPs. PEG modification and the protein corona reduced the uptake of NPs by macrophages but exerted little influence on B16 cell. Among the NPs with different lipid core, the MLCD NPs showed a lower macrophages cell uptake but higher B16 cell uptake, suggesting a longer circulation time in blood but higher cancer cell internalization. This work shed light on the interactions between MLCD NPs and proteins, which is significant for application as nanocarriers with improved biological efficacy.
基于二酰基甘油的纳米颗粒是很有前途的生物活性递送系统。然而，对它们与生物实体相互作用的有限理解限制了它们的临床应用。本研究调查了中链和长链二酰基甘油（MLCD）基固体脂质纳米颗粒（NPs）与聚氧乙烯硬脂酸酯（PEG）修饰后的蛋白质冠，并与甘油三硬脂酸酯（TG）和十六烷棕榈酸酯（CP）纳米颗粒进行了比较。牛血清白蛋白（BSA）通过疏水相互作用和氢键与 MLCD NPs 形成了蛋白质冠，导致α-螺旋减少，β-折叠增加，色氨酸残基微环境发生变化。由于较高的脂质亲水性，MLCD NPs 与 BSA 的亲和力远低于 TG 和 CP NPs，并且较大 NPs 的 BSA 结合常数增加。PEG 修饰和蛋白质冠降低了巨噬细胞对 NPs 的摄取，但对 B16 细胞几乎没有影响。在具有不同脂质核心的 NPs 中，MLCD NPs 对巨噬细胞的摄取较低，但对 B16 细胞的摄取较高，这表明在血液中的循环时间更长，但癌细胞内化更高。这项工作揭示了 MLCD NPs 与蛋白质之间的相互作用，这对于作为具有改善的生物功效的纳米载体的应用具有重要意义。
