Fabrication of novel polysaccharides and glycerol monolaurate based camellia oil composite oleogel: Application in wound healing promotion.

In this study, a novel camellia oil composite oleogel (SX@G-CO) was prepared by a combination of direct dispersion and emulsion-templated methods using polysaccharides (sodium alginate and xanthan gum, ratio in 4:6) as oleogelators and glycerol monolaurate (GML, 7 wt%) as gel-enhancer. The comparative experiments revealed that the polysaccharides could effectively enhance the densification of the three-dimensional network structure of the oleogel through hydrogen bonding and electrostatic interactions, and significantly improve its thermal stability, rheological properties (adhesive strength 49,243.6 mPa•s, viscosity recovery rate 94.6 %) and oil binding capacity (80.6 %). The introduction of GML further enriched the crystal diversity of the oleogel and imparted excellent antimicrobial ability (nearly 100 % inhibition effect on E.coli). Furthermore, the in vitro experiments demonstrated that the synergistic effect of polysaccharides and GML significantly enhanced the anti-inflammatory, antioxidant, cell migration and proliferation abilities of SX@G-CO oleogel compared with GML-CO and SX-CO oleogels. In addition, SX@G-CO oleogel has also been demonstrated to effectively promote full-thickness burn healing in mice by reducing bacterial infection and inflammatory response, regulating free radical levels, and promoting neovascularization in vivo, with effects comparable to marketed ointment. SX@G-CO oleogel as a bioactive molecule-polysaccharides composite has potential clinical application in burn wound repair.