Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, Xi'an, Shaanxi 710127, China.
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, Xi'an, Shaanxi 710127, China.
Int J Biol Macromol. 2024 Nov;281(Pt 1):136239. doi: 10.1016/j.ijbiomac.2024.136239. Epub 2024 Oct 2.
In this study, a novel camellia oil composite oleogel (SX@G-CO) was prepared by a combination of direct dispersion and emulsion-templated methods using polysaccharides (sodium alginate and xanthan gum, ratio in 4:6) as oleogelators and glycerol monolaurate (GML, 7 wt%) as gel-enhancer. The comparative experiments revealed that the polysaccharides could effectively enhance the densification of the three-dimensional network structure of the oleogel through hydrogen bonding and electrostatic interactions, and significantly improve its thermal stability, rheological properties (adhesive strength 49,243.6 mPa•s, viscosity recovery rate 94.6 %) and oil binding capacity (80.6 %). The introduction of GML further enriched the crystal diversity of the oleogel and imparted excellent antimicrobial ability (nearly 100 % inhibition effect on E.coli). Furthermore, the in vitro experiments demonstrated that the synergistic effect of polysaccharides and GML significantly enhanced the anti-inflammatory, antioxidant, cell migration and proliferation abilities of SX@G-CO oleogel compared with GML-CO and SX-CO oleogels. In addition, SX@G-CO oleogel has also been demonstrated to effectively promote full-thickness burn healing in mice by reducing bacterial infection and inflammatory response, regulating free radical levels, and promoting neovascularization in vivo, with effects comparable to marketed ointment. SX@G-CO oleogel as a bioactive molecule-polysaccharides composite has potential clinical application in burn wound repair.
在这项研究中，通过直接分散和乳液模板法相结合，使用多糖（海藻酸钠和黄原胶，比例为 4:6）作为油凝胶剂和甘油单月桂酸酯（GML，7wt%）作为凝胶增强剂，制备了一种新型的茶花油复合油凝胶（SX@G-CO）。对比实验表明，多糖可以通过氢键和静电相互作用有效地增强油凝胶三维网络结构的致密化，并显著提高其热稳定性、流变性能（粘附强度 49,243.6mPa•s，粘度恢复率 94.6%）和油结合能力（80.6%）。GML 的引入进一步丰富了油凝胶的晶体多样性，并赋予其优异的抗菌能力（对大肠杆菌的抑制率接近 100%）。此外，体外实验表明，与 GML-CO 和 SX-CO 油凝胶相比，多糖和 GML 的协同作用显著增强了 SX@G-CO 油凝胶的抗炎、抗氧化、细胞迁移和增殖能力。此外，SX@G-CO 油凝胶还通过减少细菌感染和炎症反应、调节自由基水平以及促进体内新血管生成，有效促进了小鼠全层烧伤的愈合，其效果可与市售软膏相媲美。作为一种生物活性分子-多糖复合物的 SX@G-CO 油凝胶在烧伤创面修复中有潜在的临床应用。
