Nasr Noha N, El-Hagrassi Ali M, Ahmed Yomna R, Hamed Manal A
Department of Therapeutic Chemistry, National Research Centre, Dokki, Giza, Egypt.
Phytochemistry and Plant Systematic Department, National Research Centre, 33 El-Buhouth St., Dokki, Giza, Egypt.
Chem Biodivers. 2025 Feb;22(2):e202401960. doi: 10.1002/cbdv.202401960. Epub 2024 Nov 14.
This study investigated the therapeutic effect of Conocarpus erectus leaves methanolic extract against AlCl -induced Alzheimer's disease (AD) in rats comparing with Donepezil-hydrochloride as a reference drug. The bioactive compounds of C. erectus leaves were isolated and identified by GC/MS and LC-ESI-MS analysis. Malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), amyloid-β-peptide (Aβ-peptide), tau protein, acetylcholinesterase (AChE), serotonin (5-HT), dopamine (DA) and nor-adrenaline (NE) levels were estimated. The neuromuscular strength, memory behavior and histopathological examination of cerebral cortex region were also conducted. Forty-three compounds were characterized from the non-polar fraction of C. erectus L. leaves extract and nineteen compounds were identified from the defatted extract. AlCl- induction caused significant elevation of brain oxidative stress, Aβ-peptide, tau protein, IL-6, TNF-α and AChE levels. A significant decrease in 5-HT, ND and DA levels were noticed. Additionally, AlCl reduced neuromuscular strength and compromised memory function. Treatment of AlCl3- induced rats with C. erectuse extract ameliorated these selected parameters by variable degrees. In conclusion, C. erectus protects against AlCl- induced AD in rats through its antioxidant, anti-inflammatory, and antineural damage. [Correction added on 3 December 2024, after first online publication: The term "antineutron" was corrected to "antineural" in the preceding sentence.]. It could be considered as a new nutraceutical agent for attenuating symptoms associated with Alzheimer's disease.
本研究调查了海榄雌叶甲醇提取物对氯化铝诱导的大鼠阿尔茨海默病（AD）的治疗效果，并与盐酸多奈哌齐作为参比药物进行比较。通过气相色谱/质谱联用（GC/MS）和液相色谱-电喷雾电离质谱（LC-ESI-MS）分析分离并鉴定了海榄雌叶的生物活性化合物。测定了丙二醛（MDA）、还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、淀粉样β肽（Aβ肽）、tau蛋白、乙酰胆碱酯酶（AChE）、5-羟色胺（5-HT）、多巴胺（DA）和去甲肾上腺素（NE）的水平。还进行了神经肌肉力量、记忆行为以及大脑皮质区域的组织病理学检查。从海榄雌叶提取物的非极性部分鉴定出43种化合物，从脱脂提取物中鉴定出19种化合物。氯化铝诱导导致脑氧化应激、Aβ肽、tau蛋白、IL-6、TNF-α和AChE水平显著升高。5-HT、ND和DA水平显著降低。此外，氯化铝降低了神经肌肉力量并损害了记忆功能。用海榄雌提取物治疗氯化铝诱导的大鼠可不同程度地改善这些选定参数。总之，海榄雌通过其抗氧化、抗炎和抗神经损伤作用保护大鼠免受氯化铝诱导的AD侵害。[2024年12月3日首次在线发表后添加的更正：前一句中的“antineutron”一词更正为“antineural”。]它可被视为一种减轻与阿尔茨海默病相关症状的新型营养保健剂。
