正常成纤维细胞和癌相关成纤维细胞的比较转录组学。
Comparative transcriptome of normal and cancer-associated fibroblasts.
机构信息
Centre for Human Genetics, Bengaluru, India.
Manipal Academy of Higher Education, Manipal, India.
出版信息
BMC Cancer. 2024 Oct 5;24(1):1231. doi: 10.1186/s12885-024-13006-x.
BACKGROUND
The characteristics of a tumor are largely determined by its interaction with the surrounding micro-environment (TME). TME consists of both cellular and non-cellular components. Cancer-associated fibroblasts (CAFs) are a major component of the TME. They are a source of many secreted factors that influence the survival and progression of tumors as well as their response to drugs. Identification of markers either overexpressed in CAFs or unique to CAFs would pave the way for novel therapeutic strategies that in combination with conventional chemotherapy are likely to have better patient outcome.
METHODS
Fibroblasts have been derived from Benign Prostatic Hyperplasia (BPH) and prostate cancer. RNA from these has been used to perform a transcriptome analysis in order to get a comparative profile of normal and cancer-associated fibroblasts.
RESULTS
The study has identified 818 differentially expressed mRNAs and 17 lincRNAs between normal and cancer-associated fibroblasts. Also, 15 potential lincRNA-miRNA-mRNA combinations have been identified which may be potential biomarkers.
CONCLUSIONS
This study identified differentially expressed markers between normal and cancer-associated fibroblasts that would help in targeted therapy against CAFs/derived factors, in combination with conventional therapy. However, this would in future need more experimental validation.
背景
肿瘤的特征在很大程度上取决于其与周围微环境（TME）的相互作用。TME 由细胞和非细胞成分组成。癌相关成纤维细胞（CAFs）是 TME 的主要组成部分。它们是许多分泌因子的来源，这些因子影响肿瘤的存活和进展以及对药物的反应。鉴定在 CAFs 中过度表达或 CAFs 特有的标志物将为新的治疗策略铺平道路，这些策略与常规化疗相结合可能会带来更好的患者预后。
方法
已经从良性前列腺增生（BPH）和前列腺癌中分离出成纤维细胞。使用这些细胞的 RNA 进行转录组分析，以获得正常和成纤维细胞相关性纤维母细胞的比较图谱。
结果
该研究在正常和成纤维细胞相关性纤维母细胞之间鉴定出 818 个差异表达的 mRNAs 和 17 个 lincRNAs。此外，还鉴定出了 15 个潜在的 lincRNA-miRNA-mRNA 组合，它们可能是潜在的生物标志物。
结论
这项研究鉴定出了正常和成纤维细胞相关性纤维母细胞之间差异表达的标志物，这将有助于针对 CAFs/衍生因子的靶向治疗，与常规治疗相结合。然而，这在未来需要更多的实验验证。
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