Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, Dunedin, New Zealand.
Faculty of Dentistry, Universiti Teknologi MARA, Sungai Buloh Campus, Selangor, Malaysia.
J Appl Biomater Funct Mater. 2024 Jan-Dec;22:22808000241289208. doi: 10.1177/22808000241289208.
Craniofacial bone defects result from various disorders such as trauma, congenital malformations and infections. Cleft lip and palate are the most prevalent congenital craniofacial birth defect in humans. Growth factors (GFs) are soluble proteins secreted by cells that regulate various cellular processes and tissue regeneration. At present, developing three-dimensional scaffolds for delivering GFs to the site of injury has become an important aspect in craniofacial bone regeneration. This study aims to develop a novel 3D bone substitute using lyophilized-platelet-rich fibrin (LyPRF) biocomposite scaffolds for potential application for CLP repair. Collagen (C), bioglass (BG), and LyPRF were used to fabricate a biocomposite (C-BG-LyPRF) scaffold. The physical, chemical, and biocompatibility properties of the scaffold were evaluated. The C-BG-LyPRF scaffold demonstrated a mean pore diameter of 146 µm within a porosity of 87.26%. The FTIR spectra verified the presence of am-ide I, II, and III functional groups. The inorganic phase of the C-BG-LyPRF scaffold was composed of sodium, calcium, silicon, and phosphorus, as determined by EDX analysis. Furthermore, C-BG-LyPRF scaffold was biocompatible with MC3T3-E1 cells in both the Live/Dead and prolif-eration assays. Data demonstrate the developed C-BG-LyPRF scaffold exhibits biomimetic and biocompatibility properties, establishing it as a promising biomaterial for craniofacial regeneration.
颅面骨缺损是由创伤、先天畸形和感染等多种疾病引起的。唇腭裂是人类最常见的先天性颅面出生缺陷。生长因子（GFs）是细胞分泌的可溶性蛋白，可调节多种细胞过程和组织再生。目前，开发用于将 GFs 递送到损伤部位的三维支架已成为颅面骨再生的一个重要方面。本研究旨在使用冻干富血小板纤维蛋白（LyPRF）生物复合材料支架开发一种新型的 3D 骨替代物，用于 CLP 修复的潜在应用。使用胶原（C）、生物玻璃（BG）和 LyPRF 来制备生物复合材料（C-BG-LyPRF）支架。评估了支架的物理、化学和生物相容性特性。C-BG-LyPRF 支架的平均孔径为 146µm，孔隙率为 87.26%。FTIR 光谱证实存在酰胺 I、II 和 III 官能团。EDX 分析表明，C-BG-LyPRF 支架的无机相由钠、钙、硅和磷组成。此外，C-BG-LyPRF 支架在 Live/Dead 和增殖测定中均与 MC3T3-E1 细胞具有生物相容性。数据表明，所开发的 C-BG-LyPRF 支架具有仿生和生物相容性，是一种很有前途的颅面再生生物材料。
