γ-干扰素对结核性胸腔积液的诊断价值。

Diagnostic value of IFN-gamma in tuberculous pleural effusion.

机构信息

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Anhui Medical University, Anhui Province Public Health Clinical Center, HeFei, Anhui, China.

出版信息

Cytokine. 2024 Dec;184:156773. doi: 10.1016/j.cyto.2024.156773. Epub 2024 Oct 5.

DOI:10.1016/j.cyto.2024.156773

PMID:39369468

Abstract

BACKGROUND

Simple, rapid, and accurate diagnosis of tuberculous pleural effusion (TPE) remains challenging. This study aimed to determine the accuracy of IFN-γ in diagnosing TPE.

METHODS

We quantified the expression of interferon-gamma (IFN-γ) in blood (B), adenosine deaminase (ADA), and IFN-γ in pleural effusions (PE) from 25 TPE patients and 31 non-TPE patients using a combination of immunological assays and flow cytometric analysis. The diagnostic performance of these three biomarkers was evaluated using receiver operating characteristic (ROC) curves.

RESULTS

We found that IFN-γ levels in blood and pleural fluid were higher in the TPE group than in the non-TPE group. The mean concentration of IFN-γ in pleural fluid of the TPE group was 3140.90 (1817.94, 6611.05) pg/mL, while that of the non-TPE group was 4.91 (0.69, 8.6) pg/mL), and the difference was statistically significant (z = 6.39, P < 0.001). The mean blood IFN-γ was 40.19 (16.45, 59.08) pg/mL in the TPE group and 2.76 (1.96, 6.02) pg/mL in the non-TPE group, which was statistically different (z = 5.12, P < 0.001). The area under the ROC curve (AUC) for pleural fluid IFN-γ, blood IFN-γ, and ADA were 0.999 (95 % CI: 0.994-1.00), 0.901 (95 % CI: 0.798-1.00) and 0.996 (95 % CI: 0.987-1.00), respectively.

CONCLUSION

This study confirms that IFN-γ has high diagnostic validity in patients with TPE and can potentially be an excellent biomarker.

摘要

背景

简单、快速、准确地诊断结核性胸腔积液（TPE）仍然具有挑战性。本研究旨在确定干扰素-γ在诊断 TPE 中的准确性。

方法

我们使用免疫测定和流式细胞术分析相结合的方法，对 25 例 TPE 患者和 31 例非 TPE 患者的血液（B）、腺苷脱氨酶（ADA）和胸腔积液（PE）中的干扰素-γ（IFN-γ）表达进行定量。使用接收者操作特征（ROC）曲线评估这三种生物标志物的诊断性能。

结果

我们发现 TPE 组的血液和胸腔积液中的 IFN-γ 水平高于非 TPE 组。TPE 组胸腔积液中 IFN-γ 的平均浓度为 3140.90（1817.94，6611.05）pg/ml，而非 TPE 组为 4.91（0.69，8.6）pg/ml），差异具有统计学意义（z=6.39，P<0.001）。TPE 组血液 IFN-γ 平均为 40.19（16.45，59.08）pg/ml，而非 TPE 组为 2.76（1.96，6.02）pg/ml），差异具有统计学意义（z=5.12，P<0.001）。胸腔积液 IFN-γ、血液 IFN-γ 和 ADA 的 ROC 曲线下面积（AUC）分别为 0.999（95%CI：0.994-1.00）、0.901（95%CI：0.798-1.00）和 0.996（95%CI：0.987-1.00）。