基于网络药理学研究苦参花提取物治疗 LPS 诱导的急性肺炎的作用机制。
Investigating mechanisms of Sophora davidii (Franch.) skeels flower extract in treating LPS-induced acute pneumonia based on network pharmacology.
机构信息
Jiangxi Province Key Laboratory of Pharmacology of Traditional Chinese Medicine, Gannan Medical University, Ganzhou, Jiangxi, 341000, China.
Department of Pharmacy, Xiangyang No. 1 People's Hospital, Hubei University of Medicine, Xiangyang, 441100, China.
出版信息
J Ethnopharmacol. 2025 Jan 30;337(Pt 2):118914. doi: 10.1016/j.jep.2024.118914. Epub 2024 Oct 5.
ETHNOPHARMACOLOGICAL RELEVANCE
In TCM opinion, most of pneumonia is related to "lung heat". Sophora davidii (Franch.) Skeels flower was first documented in "Guizhou Herbal Medicine", and was recorded as having functions of clearing heat, detoxifying, and cooling blood. It can be used to treat lung heat cough.
AIM OF THE STUDY
To investigate main mechanisms of Sophora davidii flower extract (SDFE) in Treating LPS-induced acute Pneumonia.
MATERIALS AND METHODS
Acute pneumonia models on BEAS-2B cells and rats were established using LPS. The rat model was used to verified the protective effects of SDFE through HE staining, lung tissue W/D ratio assay, white blood cell count analysis, and ammonia-induced coughing test. Network pharmacology was applied to predict the active compounds, core targets and main pathways of SDFE in treating acute pneumonia. Western Blot and ELISA kits were employed to validate representative proteins in selected pathway in vivo and in vitro.
RESULTS
HE staining, lung tissue W/D ratio assay, white blood cell count analysis, and ammonia-induced coughing test showed SDFE could improve pathological features (leukocyte infiltration, pulmonary edema, lung injury and cough). Network pharmacology indicated MAPK/NF-κB pathway was the most relevant pathway. SDFE could significantly inhibit the expression of Fos and Jun, and the phosphorylation levels of p38, ERK, JNK, NF-κB and IκB. It also down-regulated the expression of pro-inflammatory factors (TNF-α, IL-6 and IL-1β).
CONCLUSIONS
SDFE can exert protective effects against acute pneumonia through the MAPK/NF-κB signaling pathway.
草药学相关性
在中医理论中，大多数肺炎与“肺热”有关。苦参花最早在《贵州草药》中被记载，具有清热、解毒、凉血的功能。可用于肺热咳嗽。
研究目的
探讨苦参花提取物（SDFE）治疗脂多糖诱导的急性肺炎的主要机制。
材料与方法
采用 LPS 建立 BEAS-2B 细胞和大鼠急性肺炎模型。通过 HE 染色、肺组织湿重/干重比测定、白细胞计数分析和氨诱导咳嗽试验，验证 SDFE 对大鼠模型的保护作用。采用网络药理学预测 SDFE 治疗急性肺炎的活性化合物、核心靶点和主要途径。采用 Western Blot 和 ELISA 试剂盒验证体内和体外选定途径的代表性蛋白。
结果
HE 染色、肺组织湿重/干重比测定、白细胞计数分析和氨诱导咳嗽试验表明 SDFE 可改善病理特征（白细胞浸润、肺水肿、肺损伤和咳嗽）。网络药理学表明 MAPK/NF-κB 通路是最相关的通路。SDFE 能显著抑制 Fos 和 Jun 的表达，以及 p38、ERK、JNK、NF-κB 和 IκB 的磷酸化水平。它还下调了促炎因子（TNF-α、IL-6 和 IL-1β）的表达。
结论
SDFE 通过 MAPK/NF-κB 信号通路对急性肺炎发挥保护作用。
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