Minasyan Alexander S, Peacey Merlin, Allen Te'Kara, Nesterova Irina V
Department of Chemistry and Biochemistry, Northern Illinois University, DeKalb, IL 60115, USA.
Chembiochem. 2024 Dec 16;25(24):e202400647. doi: 10.1002/cbic.202400647. Epub 2024 Nov 11.
I-motifs are non-canonical DNA structures with recognized biological significance and a proven utility in material engineering. Consequently, understanding and control of i-motif properties is essential to sustain progress across both disciplines. In this work, we systematically investigate how proximity to the most common form of DNA, a double-stranded duplex, influences the thermodynamic and kinetic properties of adjacent i-motifs. We demonstrate that double-stranded stems in i-motif loops promote kinetic trapping of very stable and persistent partially folded conformations. Further, we investigate pathways toward rational control over a folding topology makeup.
i-基序是非经典的DNA结构，具有公认的生物学意义，并且在材料工程中具有已证实的实用性。因此，了解和控制i-基序的特性对于这两个学科的持续发展至关重要。在这项工作中，我们系统地研究了与最常见的DNA形式——双链双螺旋——的接近程度如何影响相邻i-基序的热力学和动力学特性。我们证明，i-基序环中的双链茎促进了非常稳定和持久的部分折叠构象的动力学捕获。此外，我们研究了对折叠拓扑结构组成进行合理控制的途径。
