Mito M
Nihon Geka Gakkai Zasshi. 1985 Sep;86(9):993-6.
Our previous experiment presented that isolated adult rat hepatocytes survived and proliferated markedly and they reconfigured macroscopic hepatic tissue occupying more than 50% of the spleen 27 months after transplantation. On the basis of the results, we have emphasized that the hepatized spleen provides many fundamental and clinical interests on hepatology. The morphlogical studies of the recomposed hepatic tissue revealed almost normal architecture of the liver except the lack of bile duct system. Prolongation of the survival time was obtained in rats with hepatized spleen which underwent a portacaval shunt several months earlier compared with animals without hepatized spleen, when the rats were completely devascularized. That means that the hepatized spleen can act as an auxillary liver. For the purpose of applying this experimental model clinically, liver cell autotransplantation was performed using dog and monkey. These resulted in recomposed hepatic tissue in the monkey's spleen 15 months after transplantation, but no survival of the transplanted hepatocytes in dogs. In human, we have been developed a new hepatocyte isolation technique and we can obtain approximately 10(6) isolated human hepatocytes (viability 55-86%) from the partially resected liver with the hand-made multiperfusion system. Finally, future problems of clinical application of the intrasplenic hepatocyte transplant were mentioned especially about the idea of the liver cell bank.
我们之前的实验表明，分离出的成年大鼠肝细胞能够显著存活并增殖，并且在移植27个月后，它们重新构建了占据脾脏超过50%的宏观肝组织。基于这些结果，我们强调肝化脾脏在肝脏学方面具有许多基础和临床研究价值。对重组肝组织的形态学研究显示，除了缺乏胆管系统外，肝脏结构几乎正常。与没有肝化脾脏的动物相比，几个月前接受门腔分流术且完全去血管化的肝化脾脏大鼠的存活时间得到了延长。这意味着肝化脾脏可以起到辅助肝脏的作用。为了将这个实验模型应用于临床，我们使用狗和猴子进行了肝细胞自体移植。这些实验结果表明，移植15个月后，猴子脾脏中出现了重组肝组织，但狗体内移植的肝细胞没有存活。在人类身上，我们开发了一种新的肝细胞分离技术，通过手工多灌注系统，我们可以从部分切除的肝脏中获得大约10(6)个分离的人肝细胞（活力为55 - 86%）。最后，文中提到了脾内肝细胞移植临床应用未来面临的问题，特别是关于肝细胞库的设想。
