MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences/Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution, Ocean University of China, Qingdao, Shandong/Sanya, Hainan, 266100/572025, China.
Hebei Xinhai Aquatic Biotechnology Co, Ltd, Cangzhou, 061101, China.
BMC Genomics. 2024 Oct 7;25(1):934. doi: 10.1186/s12864-024-10863-5.
The genetic basis underlying spawning abilities in the Pacific white shrimp, Penaeus vannamei, remains largely unexplored. To investigate genetic variations potentially related to reproductive performance, a systematic bioinformatic analysis was conducted to identify structural variations (SVs) with different polymorphic spectra in P. vannamei with high fertility (HF) and low fertility (LF).
A total of 2,323 and 1,859 SV events were identified exclusively in the HF and LF groups, respectively. These SVs were mapped to 277 genes in the HF group and 231 genes in the LF group. Gene Ontology (GO) enrichment analysis based on SNPs (single nucleotide polymorphism) and SVs revealed several neural-related processes, suggesting the importance of neural regulation in reproduction. Notably, we identified a set of promising genes, including Cttn, Spast, Ppp4c, Spire1, Lhcgr, and Ftz-f1, which may enhance fertility in shrimp.
In conclusion, this study is the first to establish a link between SVs and reproductive traits in P. vannamei. The promising genes discovered have the potential to serve as crucial markers for enhancing reproductive traits through targeted genotyping.
太平洋白对虾（Penaeus vannamei）产卵能力的遗传基础在很大程度上仍未得到探索。为了研究可能与繁殖性能相关的遗传变异，我们对具有高繁殖力（HF）和低繁殖力（LF）的太平洋白对虾进行了系统的生物信息学分析，以鉴定具有不同多态性谱的结构变异（SV）。
共在 HF 和 LF 组中分别鉴定出 2323 个和 1859 个 SV 事件。这些 SV 映射到 HF 组中的 277 个基因和 LF 组中的 231 个基因。基于 SNP（单核苷酸多态性）和 SV 的基因本体论（GO）富集分析揭示了几个与神经相关的过程，表明神经调节在繁殖中的重要性。值得注意的是，我们鉴定了一组有前途的基因，包括 Cttn、Spast、Ppp4c、Spire1、Lhcgr 和 Ftz-f1，它们可能增强虾的繁殖力。
总之，本研究首次在太平洋白对虾中将 SV 与繁殖性状联系起来。发现的有前途的基因有可能通过靶向基因分型成为增强繁殖性状的关键标记。
