Gould A R
J Cell Sci. 1979 Oct;39:235-45. doi: 10.1242/jcs.39.1.235.
Two methods, which relate grain number to cell cycle phase in Feulgen-stained autoradiographic preparations, have been developed and compared. Both methods automate grain number estimations, one by taking integrated absorbance measurements at different wavelengths, the other by measuring absorption at a single wavelength before and after chemical removal of silver grains. With tritium-labelled tobacco mosaic virus as a probe, a quantitative analysis has been made of the binding of virus particles to plant protoplasts in different compartments of the DNA replication and partition cycle. The preliminary results indicate that the quantity of virus bound by protoplasts is related to their cell cycle phase. Whilst in this case, the methods have been used with plant cells, both techniques are equally applicable to animal cells.
已开发并比较了两种在福尔根染色放射自显影片中将颗粒数与细胞周期阶段相关联的方法。两种方法都能自动估计颗粒数，一种是通过在不同波长下进行积分吸光度测量，另一种是在化学去除银颗粒前后在单一波长下测量吸光度。以氚标记的烟草花叶病毒为探针，对病毒颗粒在DNA复制和分配周期不同阶段与植物原生质体的结合进行了定量分析。初步结果表明，原生质体结合的病毒量与其细胞周期阶段有关。在这种情况下，这些方法已用于植物细胞，这两种技术同样适用于动物细胞。
