Department of Genetics, Research School of Biological Sciences, Australian National University, Canberra, Australia.
Plant Cell Rep. 1982 Oct;1(5):225-8. doi: 10.1007/BF00270242.
Protoplasts isolated from rapidly dividing cell suspension cultures of either Nicotiana sylvestris or tumor derived cultures of Crepis capillaris were fused by PEG or liposome treatments to form homokaryons. Analysis of binucleates by Feulgen microspectrophotometry, and autoradiography, has revealed that whereas fusion products of all cell cycle combinations occur, protoplasts of certain cycle phases participate in fusions more frequently than expected, and there is a slight predominance of "like-with-like" cycle combinations. It is argued that this tendency towards specificity of fusion may be explained by cycle related variation in surface charge on protoplasts, and the mechanisms of action of the fusogens used.
从迅速分裂的烟草原生质体悬浮培养物或毛茛属肿瘤衍生培养物中分离出来的原生质体通过 PEG 或脂质体处理融合形成同核体。通过 Feulgen 微量分光光度法和放射自显影分析双核细胞，发现尽管发生了所有细胞周期组合的融合产物，但某些周期相的原生质体比预期更频繁地参与融合，并且存在轻微的“相似相溶”周期组合优势。有人认为，这种融合特异性的趋势可以通过原生质体表面电荷的周期相关性变化以及所使用的融合剂的作用机制来解释。
