Ndou Dakalo Lorraine, Mtolo Bonakele Patricia, Khwathisi Adivhaho, Ndhlala Ashwell Rungano, Tavengwa Nikita Tawanda, Madala Ntakadzeni Edwin
Department of Chemistry Faculty of Science Engineering and Agriculture University of Venda, Private Bag X5050, Thohoyandou 0950, South Africa.
DSI-NRF Centre of Excellence in Strong Materials School of Chemistry University of the Witwatersrand, Johannesburg 2050, South Africa.
Int J Anal Chem. 2024 Sep 27;2024:2681595. doi: 10.1155/2024/2681595. eCollection 2024.
Herein, a micro-solid-phase extraction (SPE) method was developed using a pipette tip for rutin extraction, employing activated hollow carbon nanospheres (HCNSs) as the sorbent. Characterization of the activated carbon nanospheres through TGA, FTIR, and SEM analysis confirmed the success of the activation process. The study demonstrated the efficacy of PT-SPE in rutin extraction under pH 2 conditions with a standard concentration of 2 mg·L. The optimal mass of HCNSs was found to be 2 mg, and a loading volume of 500 L resulted in the maximum recovery of rutin. Propan-2-ol was the best elution solvent with 15 aspirating/dispensing cycles. The correlation of determination ( ) for the calibration curve was found to be 0.9991, and the LOD and LOQ values were 0.604 and 1.830 mg·L, respectively. The applicability of the method was demonstrated by extracting rutin from a complex leaf extract with the relative standard deviation (RSD) of 3.26%, thereby validating this method as feasible for the extraction of useful bioactive compounds from complex plant samples.
在此，开发了一种使用移液器吸头的微固相萃取（SPE）方法用于芦丁提取，采用活化空心碳纳米球（HCNSs）作为吸附剂。通过热重分析（TGA）、傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）分析对活性炭纳米球进行表征，证实了活化过程的成功。该研究证明了在pH 2条件下，标准浓度为2 mg·L时，移液器吸头微固相萃取（PT-SPE）在芦丁提取中的有效性。发现HCNSs的最佳质量为2 mg，500 μL的上样体积导致芦丁的最大回收率。丙-2-醇是最佳洗脱溶剂，经过15次抽吸/分配循环。校准曲线的测定相关系数（ ）为0.9991，检测限（LOD）和定量限（LOQ）值分别为0.604和1.830 mg·L。通过从复杂的叶提取物中提取芦丁证明了该方法的适用性，相对标准偏差（RSD）为3.26%，从而验证了该方法对于从复杂植物样品中提取有用生物活性化合物是可行的。
