Jiang Chuandong, Peng Fuyu, Zhang Li, Zhang Yuqin, Wang Jie, Li Junmin, Cui Binghui, Cao Changdai, Wang Chengqiang, Qin Yunlei, Wang Ran, Zhao Zongpeng, Jiang Jiazhu, Yang Mingfeng, Sun Mingming, Yang Long, Zhang Qiang
College of Plant Protection, Shandong Agricultural University, Tai'an, China.
Shandong China Tobacco Industry Co., Ltd., Jinan, China.
Front Microbiol. 2024 Sep 20;15:1457624. doi: 10.3389/fmicb.2024.1457624. eCollection 2024.
Plant growth, crop yield, and pest and disease control are enhanced by PGPR (Plant growth promoting rhizobacteria), which are beneficial microorganisms found in a close symbiosis with plant roots. Phytohormones are secreted, nutrient uptake is improved, and soil properties along with the microbiological environment are regulated by these microorganisms, making them a significant focus in agricultural research. In this study, the efficient PGPR strain T1 was isolated and screened from tobacco inter-root soil, and identified and confirmed by ITS sequencing technology. Tobacco growth indicators and soil property changes were observed and recorded through potting experiments. The activities of key enzymes (e.g., sucrase, catalase, urease) in soil were further determined. High-throughput sequencing technology was utilized to sequence the soil microbial community, and combined with macro-genomics analysis, the effects of T1 strain on soil microbial diversity and metabolic pathways were explored. Following the application of T1, significant improvements were observed in the height, leaf length, and width of tobacco plants. Furthermore, the physical and chemical properties of the soil were notably enhanced, including a 26.26% increase in phosphorus availability. Additionally, the activities of key soil enzymes such as sucrase, catalase, and urease were significantly increased, indicating improved soil health and fertility. Comprehensive joint microbiomics and macrogenomics analyses revealed a substantial rise in the populations of beneficial soil microorganisms and an enhancement in metabolic pathways, including amino acid metabolism, synthesis, and production of secondary metabolites. These increase in beneficial microorganisms and the enhancement of their metabolic functions are crucial for plant growth and soil fertility. This study provides valuable references for the development of innovative microbial fertilizers and offers programs for the sustainable development of modern agriculture.
植物根际促生细菌（PGPR）能促进植物生长、提高作物产量并控制病虫害，这类有益微生物与植物根系紧密共生。这些微生物会分泌植物激素，改善养分吸收，并调节土壤性质及微生物环境，因此成为农业研究的重要焦点。在本研究中，从烟草根际土壤中分离并筛选出高效PGPR菌株T1，并通过ITS测序技术进行鉴定和确认。通过盆栽试验观察并记录烟草生长指标和土壤性质变化。进一步测定了土壤中关键酶（如蔗糖酶、过氧化氢酶、脲酶）的活性。利用高通量测序技术对土壤微生物群落进行测序，并结合宏基因组分析，探究T1菌株对土壤微生物多样性和代谢途径的影响。施用T1后，烟草植株的株高、叶长和叶宽均有显著提高。此外，土壤理化性质显著增强，有效磷含量增加了26.26%。此外，土壤中蔗糖酶、过氧化氢酶和脲酶等关键酶的活性显著提高，表明土壤健康状况和肥力得到改善。综合联合微生物组学和宏基因组学分析表明，有益土壤微生物数量大幅增加，代谢途径增强，包括氨基酸代谢、次生代谢产物的合成和产生。这些有益微生物数量的增加及其代谢功能的增强对植物生长和土壤肥力至关重要。本研究为创新型微生物肥料的开发提供了有价值的参考，并为现代农业的可持续发展提供了方案。
