Reagan M E, Robb M, Bornstein I, Niday E G
Thromb Res. 1985 Oct 1;40(1):1-9. doi: 10.1016/0049-3848(85)90344-5.
Tissue plasminogen activator (tPA) was purified from serum-supplemented culture media in a single-step immunoaffinity chromatographic procedure. This was done by producing a monoclonal antibody to tissue plasminogen activator. The antibody is a murine IgG1, kappa light chain molecule. The antibody was coupled to CNBr-activated Sepharose 4B. Tissue plasminogen activator was isolated from serum supplemented tissue culture media by immunoaffinity chromatography using a relatively high ionic strength equilibration buffer and an acidic elution buffer.
组织型纤溶酶原激活剂（tPA）通过一步免疫亲和色谱法从添加血清的培养基中纯化得到。这是通过制备针对组织型纤溶酶原激活剂的单克隆抗体来实现的。该抗体是一种鼠源IgG1、κ轻链分子。该抗体与溴化氰活化的琼脂糖4B偶联。使用相对高离子强度的平衡缓冲液和酸性洗脱缓冲液，通过免疫亲和色谱法从添加血清的组织培养基中分离出组织型纤溶酶原激活剂。
