Immunoaffinity purification of tissue plasminogen activator from serum-supplemented conditioned media using monoclonal antibody.

Tissue plasminogen activator (tPA) was purified from serum-supplemented culture media in a single-step immunoaffinity chromatographic procedure. This was done by producing a monoclonal antibody to tissue plasminogen activator. The antibody is a murine IgG1, kappa light chain molecule. The antibody was coupled to CNBr-activated Sepharose 4B. Tissue plasminogen activator was isolated from serum supplemented tissue culture media by immunoaffinity chromatography using a relatively high ionic strength equilibration buffer and an acidic elution buffer.