Sato K, Ikeda T, Katami K, Ogawa H
Acta Pathol Jpn. 1985 Nov;35(6):1375-83. doi: 10.1111/j.1440-1827.1985.tb01435.x.
Monoclonal antibody (MoAb) to rat liver plasma membranes was prepared by hybridization of mouse immune lymphocytes with mouse myeloma cells, and was identified by the immunodiffusion method in a fraction of IgM secreted from the hybridoma thus obtained. In indirect immunofluorescence tests, specific fluorescence was detected only on the surface of rat hepatocytes, but neither on the cells from other organs of the rat nor on the hepatocytes of other species of animals, suggesting that the antibody may be organ- and species-specific. When the primary culture rat hepatocytes, labelled with isotopic chromium (51Cr), were treated with the MoAb together with complement, a specific release of 51Cr from the cells was found shortly after treatment, accompanied with bubbling of the cell membranes, and a significant release of 51Cr was observed at an MoAb concentration of 15 micrograms/ml or more. Without complement, or with inactivated complement, these reactions were not observed. These facts suggest strongly that the cell surface of the hepatocytes was damaged by the MoAb in the presence of complement.
通过将小鼠免疫淋巴细胞与小鼠骨髓瘤细胞杂交制备了抗大鼠肝细胞膜的单克隆抗体（MoAb），并通过免疫扩散法在由此获得的杂交瘤分泌的一部分IgM中进行了鉴定。在间接免疫荧光试验中，仅在大鼠肝细胞表面检测到特异性荧光，而在大鼠其他器官的细胞或其他动物物种的肝细胞表面均未检测到，这表明该抗体可能具有器官和物种特异性。当用同位素铬（51Cr）标记的原代培养大鼠肝细胞与MoAb及补体一起处理时，处理后不久发现细胞有特异性的51Cr释放，同时伴有细胞膜起泡，在MoAb浓度为15微克/毫升或更高时观察到51Cr有显著释放。没有补体或用灭活补体时，未观察到这些反应。这些事实有力地表明，在补体存在下，MoAb损伤了肝细胞的细胞表面。
