College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Int J Biol Macromol. 2024 Nov;281(Pt 3):136324. doi: 10.1016/j.ijbiomac.2024.136324. Epub 2024 Oct 5.
In this study, the electrospun core-shell nanofibers of zein/pullulan stabilized bilayer emulsions before and after genipin crosslinking were fabricated. The experimental results indicated that the addition of pullulan increased the apparent viscosity and elastic modulus of the bilayer emulsions, which was further increased after the chemical crosslinking of genipin. The nanofiber diameter increased from 102.9 nm to 169.9 nm with the increasing ratio of pullulan, but increased significantly to a range of 405.6-708.0 nm after genipin crosslinking. The electrospun nanofiber films of crosslinked emulsions had higher thermal stability and stronger water stability. The FTIR result proved the existence of hydrogen bond interaction between the zein, pullulan, and genipin molecules. In addition, before and after crosslinking, the encapsulation efficiency of electrospun fiber films for camellia oil was >77.68 %, and the maximum encapsulation efficiency could reach 87.94 %, and there was no significant change during the 7-day storage period, showing good stability. These research results can provide a theoretical basis for the encapsulation of hydrophobic active substances in zein-based emulsion electrospun core-shell nanofibers.
在这项研究中，制备了经过京尼平交联前后的玉米醇溶蛋白/普鲁兰稳定的双层乳液电纺核壳纳米纤维。实验结果表明，普鲁兰的添加增加了双层乳液的表观粘度和弹性模量，而经过京尼平的化学交联后进一步增加。随着普鲁兰比例的增加，纳米纤维直径从 102.9nm 增加到 169.9nm，但经过京尼平交联后显著增加到 405.6-708.0nm 的范围。交联乳液的电纺纳米纤维膜具有更高的热稳定性和更强的耐水性。傅里叶变换红外光谱（FTIR）结果证明了玉米醇溶蛋白、普鲁兰和京尼平分子之间氢键相互作用的存在。此外，交联前后，电纺纤维膜对茶籽油的包封效率均>77.68%，最大包封效率可达 87.94%，在 7 天的储存期内没有明显变化，表现出良好的稳定性。这些研究结果可为玉米醇溶蛋白基乳液电纺核壳纳米纤维中疏水性活性物质的包封提供理论依据。
