Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
University of Chinese Academy of Sciences, Beijing, China.
Nat Commun. 2024 Oct 8;15(1):8693. doi: 10.1038/s41467-024-52945-8.
Translation, a fundamental process regulating cellular growth and proliferation, relies on functional ribosomes. As sessile organisms, plants have evolved adaptive strategies to maintain a delicate balance between growth and stress response. But the underlying mechanisms, particularly on the translational level, remain less understood. In this study, we revealed the mechanisms of AtPRMT3-RPS2B in orchestrating ribosome assembly and managing translational regulation. Through a forward genetic screen, we identified PDCD2-D1 as a suppressor gene restoring abnormal development and ribosome biogenesis in atprmt3-2 mutants. Our findings confirmed that PDCD2 interacts with AtPRMT3-RPS2B, and facilitates pre-ribosome transport through nuclear pore complex, finally ensuring normal ribosome translation in the cytoplasm. Additionally, the dysfunction of AtPRMT3-RPS2B was found to enhance freezing tolerance. Moreover, we revealed that AtPRMT3-RPS2B promotes the translation of housekeeping mRNAs while concurrently repressing stress-related mRNAs. In summary, our study sheds light on the regulatory roles of AtPRMT3-RPS2B in ribosome assembly and translational balance, enabling the trade-off between growth and stress.
翻译是调节细胞生长和增殖的基本过程，依赖于功能性核糖体。作为固着生物，植物已经进化出适应性策略来维持生长和应激反应之间的微妙平衡。但潜在的机制，特别是在翻译水平上，仍然知之甚少。在这项研究中，我们揭示了 AtPRMT3-RPS2B 在协调核糖体组装和管理翻译调控中的机制。通过正向遗传学筛选，我们鉴定出 PDCD2-D1 是一种恢复 atprmt3-2 突变体中异常发育和核糖体生物发生的抑制基因。我们的研究结果证实 PDCD2 与 AtPRMT3-RPS2B 相互作用，并通过核孔复合体促进前核糖体运输，最终确保细胞质中核糖体的正常翻译。此外，发现 AtPRMT3-RPS2B 的功能障碍可增强植物的抗冻能力。此外，我们揭示了 AtPRMT3-RPS2B 促进看家 mRNA 的翻译，同时抑制应激相关的 mRNA。总之，我们的研究揭示了 AtPRMT3-RPS2B 在核糖体组装和翻译平衡中的调节作用，使生长和应激之间能够实现权衡。
