Department of Infectious Diseases, Public Health Service (GGD) of Rotterdam-Rijnmond, Rotterdam, the Netherlands.
Department of Infectious Diseases, Public Health Service (GGD) of Amsterdam, Amsterdam, the Netherlands.
Parasit Vectors. 2024 Oct 7;17(1):419. doi: 10.1186/s13071-024-06488-y.
Scabies, an infestation of the mite Sarcoptes scabiei, has seen an increase in clinical diagnoses in the Netherlands since 2011. This study aimed to analyse PCR-positive S. scabiei skin samples through partial genome sequencing and to link findings to patient epidemiological characteristics.
Skin samples were collected from individuals in the Netherlands between January 2016 and January 2023. On the PCR-positive S. scabiei skin samples, partial mitochondrial cytochrome c oxidase subunit 1 gene (cox1) sequencing was performed to assess genetic variability. Epidemiological information was collected through interviews. We examined associations between cox1 subtypes, epidemiological factors and treatment outcomes.
Sequencing results were obtained from 128 patients, with epidemiological information available for 55 (43%) of these patients. Fifteen distinct cox1 subtypes were identified. Subtype 01 was most prevalent (45%) and present across all age groups and social settings. The remaining subtypes were less common and not consistently found in all contexts. Five clusters were identified, each with identical cox1 subtypes. Comparative analysis with GenBank sequences revealed genetic similarities with strains from Australia, the USA and China, suggesting the global distribution and transmission of specific subtypes. A substantial proportion (73%) of patients with scabies required multiple treatments to eradicate the infestation, with no subtype-related differences.
This is the first study linking S. scabiei sequencing results to patient epidemiological data. Several subtypes clustered in specific geographic regions and social contexts, underscoring localised transmission patterns. Further research with larger sample sizes is needed to enhance our understanding of the transmission of this mite. This study provides valuable insights that will strengthen scabies control efforts.
自 2011 年以来，荷兰的疥疮（由疥螨引起的寄生虫病）临床诊断病例有所增加。本研究旨在通过部分基因组测序分析 PCR 阳性的疥螨皮肤样本，并将结果与患者的流行病学特征联系起来。
本研究于 2016 年 1 月至 2023 年 1 月期间在荷兰采集了个体的皮肤样本。对 PCR 阳性的疥螨皮肤样本进行部分线粒体细胞色素 c 氧化酶亚基 1 基因（cox1）测序，以评估遗传变异性。通过访谈收集流行病学信息。我们研究了 cox1 亚型、流行病学因素和治疗结果之间的关联。
从 128 名患者中获得了测序结果，其中 55 名（43%）患者提供了流行病学信息。鉴定出 15 种不同的 cox1 亚型。亚型 01 最为普遍（45%），存在于所有年龄组和社会环境中。其余亚型较为罕见，并非在所有情况下都能发现。确定了五个聚类，每个聚类都具有相同的 cox1 亚型。与 GenBank 序列的比较分析显示，与来自澳大利亚、美国和中国的菌株具有遗传相似性，表明特定亚型在全球范围内传播和传播。相当一部分（73%）患有疥疮的患者需要多次治疗才能根除感染，而不同亚型之间没有差异。
这是首次将 S. scabiei 测序结果与患者流行病学数据联系起来的研究。几个亚型在特定的地理区域和社会环境中聚类，突显了局部传播模式。需要进一步研究更大的样本量，以提高我们对这种螨虫传播的理解。本研究提供了有价值的见解，将加强疥疮的控制工作。
