Department of Clinical Biochemistry, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
Digestive Disease Research Center, Ardabil University of Medical Sciences, Ardabil, Iran.
Genet Test Mol Biomarkers. 2024 Nov;28(11):445-451. doi: 10.1089/gtmb.2024.0302. Epub 2024 Oct 8.
Gastric cancer's (GC) cause is unknown, but its complexity indicates that, in addition to environmental factors, it may have genetic origins. Scientists are studying single-nucleotide polymorphisms (SNPs) in the antisense noncoding RNA in the INK4 locus (ANRIL) gene, which encodes a long noncoding RNA molecule. They found a link between the ANRIL gene product and some polymorphisms and GC, suggesting genetic changes may lead to precancerous conditions. In a case-control research that included 250 patients with GC and 210 controls who were age- and gender-matched, four SNPs within the ANRIL gene were genotyped. These SNPs were rs1333049, rs496892, rs2383207, and rs2151280. Tetra-primer amplification refractory mutation system-PCR was utilized to carry out the process of genotyping. It was found that the chance of developing GC was connected with three SNPs rs2151280, rs1333049, and rs496892. Nevertheless, rs2383207 did not demonstrate any meaningful connection. In addition, whereas CCTC and TTCC haplotypes were shown to be less common, certain haplotypes that contained these SNPs (TTCG, TCTC, and TTTC) displayed a considerably higher prevalence in the cancer group in comparison to the control group. This study showed novel associations between specific ANRIL gene polymorphisms (SNPs) and the risk of GC. These findings shed light on the potential role of ANRIL SNPs in GC risk and highlight the need for additional research to clarify the underlying functional processes. Understanding these functional processes might lead to developing novel diagnostic or treatment approaches for this cancer.
胃癌（GC）的病因尚不清楚，但它的复杂性表明，除了环境因素外，它可能还有遗传起源。科学家们正在研究位于 INK4 基因座的反义非编码 RNA 中的单核苷酸多态性（SNP），该基因座编码一个长非编码 RNA 分子。他们发现 ANRIL 基因产物与某些多态性和 GC 之间存在联系，这表明遗传变化可能导致癌前状态。在一项包括 250 例 GC 患者和 210 例年龄和性别匹配的对照的病例对照研究中，对 ANRIL 基因内的四个 SNP 进行了基因分型。这些 SNP 是 rs1333049、rs496892、rs2383207 和 rs2151280。采用四引物扩增受阻突变系统-PCR 进行基因分型。结果发现，GC 的发病几率与三个 SNP rs2151280、rs1333049 和 rs496892 有关。然而，rs2383207 没有表现出任何有意义的关联。此外，虽然 CCTC 和 TTCC 单倍型较少见，但含有这些 SNP 的某些单倍型（TTCG、TCTC 和 TTTC）在癌症组中比对照组更为常见。本研究显示特定 ANRIL 基因多态性（SNP）与 GC 风险之间存在新的关联。这些发现揭示了 ANRIL SNP 在 GC 风险中的潜在作用，并强调需要进一步研究以阐明潜在的功能过程。了解这些功能过程可能为开发针对这种癌症的新的诊断或治疗方法提供思路。
