Department of Biological Engineering, College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian, 361021, China.
Engineering Research Center of Natural Cosmeceuticals College of Fujian Province, Xiamen Medical College, Xiamen, Fujian, 361023, China.
Biomater Sci. 2024 Oct 22;12(21):5631-5643. doi: 10.1039/d4bm00591k.
Edible plants, rich in antioxidant compounds, offer defense against oxidative stress-induced cellular damage. However, the antioxidative benefits of edible plant-derived molecules are limited due to their instability, poor solubility, and low bioavailability. Plant-derived nanovesicles (PDNVs) have emerged as the next-generation nanotherapeutics and delivery platforms; yet, challenges including low purity, significant heterogeneity, insufficient enrichment of bioactive component and compromised therapeutic efficacy limit their application. In this study, a solvent-assisted vesicle hybridization technique was developed to engineer hybrid plant-derived nanovesicles (PDNVs), exemplified by grape and tomato-derived nanovesicles (GT-HNVs), which outperform their natural counterparts. The GT-HNVs demonstrated superior stability, enhanced radical-scavenging capabilities, and greater cellular uptake efficiency. Notably, GT-HNVs significantly reduced reactive oxygen species (ROS) levels and improved antioxidative enzyme activities in L-02 cells. Moreover, they mitigated oxidative stress-induced mitochondrial damage, restoring the membrane potential and morphology. Collectively, these findings underscore the therapeutic potential of hybrid PDNVs and offer an innovative strategy for their future research.
食用植物富含抗氧化化合物，可抵御氧化应激引起的细胞损伤。然而，由于其不稳定性、低溶解度和生物利用度低，食用植物衍生分子的抗氧化益处有限。植物衍生的纳米囊泡（PDNVs）已成为下一代纳米治疗剂和给药平台；然而，包括低纯度、显著异质性、生物活性成分富集不足和治疗效果受损在内的挑战限制了它们的应用。在这项研究中，开发了一种溶剂辅助囊泡杂交技术来构建杂交植物衍生纳米囊泡（PDNVs），以葡萄和番茄衍生的纳米囊泡（GT-HNVs）为例，其性能优于天然对应物。GT-HNVs 表现出更好的稳定性、增强的自由基清除能力和更高的细胞摄取效率。值得注意的是，GT-HNVs 显著降低了 L-02 细胞中的活性氧 (ROS) 水平并提高了抗氧化酶活性。此外，它们减轻了氧化应激引起的线粒体损伤，恢复了膜电位和形态。总之，这些发现强调了杂交 PDNVs 的治疗潜力，并为它们的未来研究提供了一种创新策略。
