College of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China.
Zhejiang Hospital, Hangzhou, Zhejiang 310013, China.
Anal Methods. 2024 Nov 14;16(44):7576-7588. doi: 10.1039/d4ay01162g.
This study aimed to establish an effective affinity-ultrafiltration coupled with mass spectrometry (AUF-MS) method to rapidly screen the active components of antithrombin in Xuefu Zhuyu decoction (XFZYD). The HPLC fingerprint of different batches of XFZYD was established to verify the stability of the compound ingredients. AUF-MS was established for the rapid screening of the active ingredients in XFZYD. Eight active ingredients were screened using AUF-MS: oxypaeoniflorin, chlorogenic acid, amygdalin, isoliquiritin, neohesperidin, hesperidin, glycyrrhizic acid and chikusetsu saponin IVa. Simultaneously, the anticoagulant effect of the active ingredients was verified by performing and experiments. The results of this study indicate that AUF-MS can be used for high-throughput screening of active components of traditional Chinese medicine compounds, thus providing a basis for the discovery of thrombin inhibitors and promoting the research progress of antithrombotic drugs. The compounds screened from XFZYD showed an inhibitory effect against thrombin and a certain antithrombotic effect.
本研究旨在建立一种有效的亲和超滤与质谱联用（AUF-MS）方法，以快速筛选血栓通饮（XFZYD）中抗凝血酶的活性成分。建立了不同批次 XFZYD 的 HPLC 指纹图谱，以验证化合物成分的稳定性。建立了 AUF-MS 快速筛选 XFZYD 中的活性成分。使用 AUF-MS 筛选出 8 种活性成分：氧化芍药苷、绿原酸、苦杏仁苷、异甘草苷、新橙皮苷、橙皮苷、甘草酸和远志皂苷 IVa。同时，通过 和 实验验证了活性成分的抗凝作用。研究结果表明，AUF-MS 可用于高通量筛选中药化合物的活性成分，为发现凝血酶抑制剂提供了依据，促进了抗血栓药物的研究进展。从 XFZYD 中筛选出的化合物对凝血酶具有抑制作用，并具有一定的抗血栓作用。
