大鼠肝实质细胞和非实质细胞对牛睾丸β-半乳糖苷酶的摄取与降解
Uptake and degradation of bovine testes beta-galactosidase by parenchymal and nonparenchymal rat liver cells.
作者信息
Blomhoff R, Blomhoff H K, Tolleshaug H, Christensen T B, Berg T
出版信息
Int J Biochem. 1985;17(12):1321-8. doi: 10.1016/0020-711x(85)90055-2.
PMID:3937748
Abstract
The plasma half-life of beta-galactosidase in rat was about 1.5 min. Ten minutes after in vivo injection, 45% of the enzyme was recovered in liver, with hepatocytes and endothelial cells as the predominant cell types responsible for uptake. In vitro uptake of beta-galactosidase in hepatocytes and nonparenchymal liver cells was saturable, Ca2+-dependent and it could be partly inhibited by mannose or alpha-methyl-mannoside.
摘要
β-半乳糖苷酶在大鼠体内的血浆半衰期约为1.5分钟。体内注射十分钟后，45%的酶在肝脏中被回收，肝细胞和内皮细胞是摄取该酶的主要细胞类型。β-半乳糖苷酶在肝细胞和非实质肝细胞中的体外摄取具有饱和性、Ca2+依赖性，并且可被甘露糖或α-甲基甘露糖苷部分抑制。
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